云南玉米大斑病群体遗传多样性的RAPD分析  

RAPD Analysis Among Isolates of Exserohilum turcicum in Maize from Yunnan Province

作　　者：姜开梅[1] 范静华[2] 刘群[2] 

机构地区：[1]云南农业职业技术学院,昆明650031 [2]云南农业大学植物病理重点实验室/农业生物多样性与病害控制教育部重点实验室,昆明650201

出　　处：《中国农学通报》2012年第27期225-229,共5页Chinese Agricultural Science Bulletin

基　　金：云南省科技攻关项目"生物多样性控制农作物病害的研究"(2001NG10)

摘　　要：为了从分子水平上了解云南玉米大斑病菌遗传变异情况,为玉米大斑病菌种群致病力分化和病害有效控制提供理论依据。采用RAPD分析技术,对采自云南省部分地区的56个大斑病菌株进行了RAPD分析。对供试菌株间的遗传距离进行聚类分析并构建系统树状图,聚类分析结果表明,供试56个菌株被划分为10个遗传聚类组,RAPD分组与菌株的地理来源无明显相关性。云南省不同地区的玉米大斑病菌株整体亲缘关系相近,但各菌株间存在遗传差异。In order to study the genetic variation of Yunnan Exserohilum turcicum from the molecular level, and provide theoretical basis for Exserohilum turcicum pathogenicity virulence differentiation and effective control of disease, Using random amplified polymorphic DNA technique, fifty-six Exserohilum turcicum isolates on maize that from some areas of Yunnan Province were tested for RAPD. Based on the distance, a dendrogram was constructed of the 56 strains tested, 10 genetic groups were formed. There was no correlation found between RAPD group and the geographic origin. The analysis showed that, some strains from one area were closely related to some from another area. Nonetheless, rich genetic variation existed among the tested strains.

关 键 词：玉米大斑病菌 RAPD 遗传多样性 

分 类 号：S432.1[农业科学—植物病理学]