葛根黄豆苷元对沙土鼠脑缺血及再灌注损伤的神经保护作用(英文)  

Neuroprotective effects of daidzein on cerebral ischemia and reperfusion in gerbils

作　　者：翁鸿博[1] 刘玉兰[2] 程能能[1] 

机构地区：[1]复旦大学药学院药理学教研室,上海201203 [2]沈阳药科大学药理学教研室,沈阳110016

出　　处：《中国临床药学杂志》2012年第5期265-270,共6页Chinese Journal of Clinical Pharmacy

基　　金：The grant of the Science and Technology Research Key Projects(No.11431920102),Science and Technology Commission of Shanghai

摘　　要：目的研究葛根黄豆苷元(DZ)对沙土鼠脑缺血及缺血再灌注损伤的保护作用。方法采用结扎沙土鼠左侧及两侧颈总动脉分别制备脑缺血及缺血再灌注损伤模型,术前20 min腹腔注射DZ(35、70 mg·kg^(-1)),双盲法记录沙土鼠脑缺血1、3、6h后卒中指数;组织学方法检查神经元的病理学损伤;脑缺血24 h及缺血10 min再灌24 h后,利用干燥称重法及原子吸收分光光度法测定脑内水、钙、钠离子的含量。结果与溶媒组相比,DZ能明显降低沙土鼠卒中指数,缓解缺血性脑神经元的损伤(P<0.05)。缺血24 h后,DZ治疗组沙土鼠脑组织内水、钙、钠含量明显低于溶媒组(P<0.05)。缺血10min再灌24 h后,与溶媒组相比,DZ也明显降低缺血再灌注沙土鼠脑组织中水、钙、钠的含量(P<0.05和P<0.01)。结论 DZ能改善沙土鼠脑缺血及缺血再灌注性脑损伤,其机制可能是减少钙、钠、水在脑细胞内的蓄积。A/M To study the protective effect of daidzein （DZ）on ischemic and reperfusion brain damage in ger- bils. METHODS The gerbil cerebral ischemia model was prepared by the left carotid artery occlusion and ischemic- reperfusion injury was produced by recirculating after the ligation of bilateral carotid artery for 10 min. DZ was adminis- trated intraperitoneally 20 min prior to the onset of ischemia and ischemic-reperfusion. Stroke index score was got by ob- serving the stroke symptom during ischemia and the neuronal injuries in the cortex were evaluated quantitatively 24 h after ischemia. The contents of water, calcium and sodium in brain in gerbils were measured at 24 h ischemia and 24 h reper- fusion following 10 min isehemia. RESULTS Stroke index score of the ischemic gerbils was diminished and the neuronal damage was markedly improved by DZ treatment. After 24 h ischemia, the brain water, calcium and sodium contents in DZ treatment group were significantly lower than those in vehicle group （ P 〈 0.05 ）. At 24 h reperfusion following 10 min ischemia, DZ treatment significantly reduced the brain water, calcium and sodium contents compared with the vehi- cle group （ P 〈 0.05 and P 〈 0.01 ）. CONCLUSION DZ exhibits protective effects on cerebral ischemia and ischemic- reperfusion injuries in gerbils, and its mechanism might be related to reducing the intracellular calcium, sodium and wa- ter accumulation.

关 键 词：葛根黄豆苷元 脑缺血再灌注 钙 钠 脑损伤 

分 类 号：R285.5[医药卫生—中药学]