射干合剂的HPLC-UV指纹图谱分析  

HPLC - UV fingerprint analysis of Shegan mixture

作　　者：唐跃年[1] 金樑[1] 张青[1] 杨传敏[1] 陆晓彤[1] 刘艳[1] 徐阿晶[1] 张健[1] 

机构地区：[1]上海交通大学医学院附属新华医院药剂科,上海200092

出　　处：《中国临床药学杂志》2012年第5期283-286,共4页Chinese Journal of Clinical Pharmacy

基　　金：上海市科学技术委员会课题(编号11DZ1972500);上海交通大学医学院科技基金(编号JYY0905)

摘　　要：目的建立射干合剂的液相色谱指纹图谱标准。方法用Inertsil ODS-3色谱柱分离样品,流动相为乙腈-0.5%磷酸(pH 2.25),梯度洗脱,流速1 mL·min^(-1),检测波长215 nm,记录射干合剂的液相色谱指纹图谱。结果射干合剂指纹图谱有17个共有峰,多数峰可以达到良好分离。结论本法具有良好的精密度、稳定性和重复性,指纹图谱的检测标准可以作为射干合剂质量标准。AiM To establish a HPLC - UV fingerprint standard of Shegan mixture. METHODS The determi- nation was performed on an Inertsil~ ODS - 3 column （250 mm 4 mm, 5 ktm） with acetonitrile - 0.5%0 phosphoric acid （pH 2.25） used as a mobile phase at a flow rate of 1.0 mL＂ min-l, with gradient elution. The detection wavelength was set at 215 nm. The HPLC - UV fingerprints of Shegan mixture were recorded. RESULTS There were 17 common peaks in HPLC - UV fingerprints of Shegan mixture. Most of them were well separated. CONCLUSION The method appears to be accurate, stable and reproducible which can be served as a quality standard of Shegan mixture.

关 键 词：指纹图谱 高效液相色谱 射干合剂 质量标准 制剂 

分 类 号：R286.0[医药卫生—中药学]