天麻素对长春新碱致大鼠肝损伤的保护作用  

Protection of gastrodin on vincristine-induced liver injury in rat

作　　者：张楚君[1] 张金芝[1] 郑卫红[1] 宫登辉[1] 王雅琴[1] 

机构地区：[1]三峡大学医学院药理教研室,湖北宜昌443002

出　　处：《实用肿瘤杂志》2012年第5期521-525,共5页Journal of Practical Oncology

基　　金：湖北省自然科学基金项目(2009CDZ036);湖北省教育厅科技研究重点资助项目(D20101208);三峡大学医学院大学生科技创新基金项目(2011XGK018)

摘　　要：目的观察天麻素对长春新碱致大鼠肝损害的影响。方法 SD大鼠随机分为5组:A组为生理盐水对照组,B组为肝损伤模型组,C～E组为天麻素低、中、高剂量组。除A组用生理盐水对照外,其他组均隔日腹腔注射长春新碱,于实验第10天开始,天麻素组每天分别加用0.6%、1.2%、2.4%3种不同浓度天麻素腹腔注射,治疗1周,A、B组用生理盐水同步对照。同时观察并记录大鼠的体重和一般状况,治疗1周后,眼球取血,检测各组大鼠血清的ALT和AST的含量,并计算AST/ALT的比值;然后用4%多聚甲醛活体灌流大鼠,取肝大叶做组织病理切片,用HE染色,观察各组大鼠肝组织及细胞的形态学结构变化。结果实验第10天体重结果显示,使用长春新碱的各组大鼠的体重增长与对照组比较不明显,但随后C～E组大鼠在应用不同剂量天麻素治疗1周后,体重有所增长,E组增长最为明显,但B组的体重不增反降。血清肝功能检测结果显示,B组ALT含量和AST/ALT比值较A组均有显著升高(P<0.05,P<0.01),而C～E组ALT含量和AST/ALT与B组比较均有不同程度降低,差异有统计学意义(P<0.01,P<0.01)。肝组织切片的形态学结果显示,A组肝细胞排列整齐,细胞膜完整,无细胞变性坏死等改变,无炎性细胞浸润等炎症反应;而B组肝细胞明显水肿、脂肪空泡样变性,甚至有明显坏死病变等改变,并可见炎性细胞浸润;但C～E组肝组织形态学变化有不同程度的改善,水肿、脂肪变性以及坏死等病理改变随剂量增加有逐渐好转的趋势,以E组改善最明显。结论长春新碱对大鼠具有肝脏毒性作用,而天麻素对其有保护作用,且呈剂量依赖性。Objective To observe the effects of gastrodin on the liver injury induced by vincristine in rat.Methods SD rats were randomly divided into 5 groups： the control group（A）,vincristine group（B）,small and middle and large dose gastrodin groups（C^E）.Except for group A,Vincristine was administered by intraperitoneal injection on alternate days in B^E groups.Then,from 10th day,gastrodin began to be administered also by intraperitoneal injection each afternoon in C^E groups and saline in A^B groups to observe and record the rat′s weight and general condition.After treatment for 1 week,blood from eyes was taken,the contents of serum ALT and AST measured,and AST/ALT counted.The rat livers were fixed by living perfusion using 4% paraformaldehyde,and hepatic tissue was sliced and dyed with HE.The changes of the structure of liver tissue and cells were observed.Results Vincristine could inhibit rat′s weight growth.But after gastrodin treatment for 1 week,the rat′s weight grew with dose-dependence.The result of serum test showed that ALT and AST/ALT were significantly increased in group B compared with group A（P0.05,P0.01）.The ALT and AST/ALT were significantly decreased in groups C^E compared with group B（P0.01,P0.01）.The morphology result showed that hepatocytes of group A was marshaling,the cell membrane was intact and no cell degeneration or necrosis was observed,and the inflammatory cells were invisible in the slice.But in the slice of group B,hepatocytes had obvious edema and degeneration liking fat vacuolus and necrosis.In slice the inflammatory cells were visible.However,the morphological structure changes of these liver tissue were improved obviously in different dose gastrodin groups compared with group B. ConclusionGastrodin has protective action on vincristine-induced liver injure in rat with dosage-dependence.

关 键 词：抗肿瘤药 药物疗法 天麻素/治疗应用 长春新碱/治疗应用 肝损伤 

分 类 号：R285.5[医药卫生—中药学]