大麻素受体CB2在机械牵张力介导的人牙周膜细胞成骨分化中的作用  

Effect of cannabinoid receptor CB2 on mechanical tensile strain-stimulated osteogenic differentiation of human periodontal ligament cells

作　　者：钱红 赵亚[2] 胡静[3] 闫英剑[4] 

机构地区：[1]第四军医大学口腔医院正畸科,陕西西安710032 [2]第四军医大学微生物学与病原生物学教研室 [3]北京军区总医院口腔科 [4]石家庄医学高等专科学校口腔系

出　　处：《中国美容医学》2012年第10期1765-1767,共3页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助项目(30900285)

摘　　要：目的:研究大麻素Ⅱ型受体(cannabinoi dreceptor Ⅱ,CB2)在机械牵张力介导的人牙周膜细胞中的表达以及成骨分化中的作用。方法:体外培养人牙周膜细胞,构建细胞-机械牵张力加载模型,施加不同大小的机械牵张力,采用Real-timePCR和细胞免疫荧光化学技术检测CB2在人牙周膜细胞中mRNA和蛋白的表达。用碱性磷酸酶(ALP)试剂盒检测机械牵张力介导的细胞ALP活性。结果:对人牙周膜细胞施加不同大小的机械牵张力24h,CB2 mRNA的表达随机械牵张力的力值增大而显著性增加(P<0.05),在18%拉伸应变率作用下表达量最高(P<0.05),此时CB2蛋白的表达显著增加。加入CB2激动剂HU-308后,施加18%拉伸应变率的机械牵张力作用于人牙周膜细胞24h,ALP活性显著性增加(P<0.05)。结论:CB2在人牙周膜细胞中的表达与机械牵张力的力值具有相关性。在机械牵张力作用下,大麻素受体CB2与其配体结合能够促进人牙周膜细胞的成骨分化,从而在正畸牙槽骨改建中发挥重要作用。Objective To investigate the expression and effect of cannabinoid receptor CB2 on mechanical tensile strain-stimulated osteogenic differentiation of human periodontal ligament cells （HPDLCs）. Methods HPDLCs were cultured in vitro and the cells were stretched by mechanical tensile strain of different magnitudes.Real-time PCR and immunofiuorescence assay were used to examine CB2 expression from mRNA to protein levels following mechanical tensile strain, respectively. The activity of alkaline phosphatase （ALP） in HPDLCs was further studied. Results There was a magnitude-dependent increase in CB2 mRNA expression following mechanical tensile strain for 2411 （P〈0.05）, with the highest level at 18% elongation. CB2 protein expression was also found to enhance at 18% elongation for 24h. After addition of CB2 agonist HU-308, the activity of ALP was up-regulated at 18% elongation for 24h （P〈0.05）. Conclusion CB2 expression was greatly related to the magnitudes of mechanical tensile strain. In addition, activation of CB2 by its agonist increased the mechanical tensile strain-stimulated osteogenic differentiation of HPDLCs. This implies that CB2 might play an important role in alveolar bone remodeling.

关 键 词：大麻素Ⅱ型受体 机械牵张力 成骨分化 牙周膜细胞 

分 类 号：Q813.1[生物学—生物工程]