cDNA-AFLP技术在果树生物技术育种上的应用  

Application of the cDNA-AFLP Technique on Biotechnology Breeding of Fruit Tree

作　　者：胡淑英[1] 张春红[1] 王小敏[1] 李维林[1] 吴文龙[1] 

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《黑龙江农业科学》2012年第10期25-28,共4页Heilongjiang Agricultural Sciences

基　　金：江苏省自然科学基金资助项目(BK2011686);江苏省农业"三新"工程资助项目[sx(2011)240];2010年江苏省"博士集聚计划"资助项目;江苏省中国科学院植物研究所青年科技创新基金资助项目(青201101)

摘　　要：cDNA-AFLP技术是近年来广泛应用于植物基因分离与表达研究的mRNA指纹图谱技术,具有可靠、高效的优点。在果树生物技术应用方面,目前该技术在第一代果树中应用居多且已取得大量成果,在第二代果树上仅有少量报道,在第三代果树上的应用处于起步阶段。在简单叙述其基本原理和特点的基础上,重点阐述其在果树基因分析研究中的应用,包括芽变突变体鉴定、基因分离、基因表达特性分析以及指纹图谱构建,并对其在果树生物技术育种研究上的应用前景进行了展望。cDNA-AFLP technique is a kind of mRNA fingerprint technology widely used in plant gene isolation and expression with reliable and efficient advantages.As to its current application in fruit tree biotechnology,the technique is mainly used in the first generation of fruit trees and has achieved many achievements,while in the second generation of fruit trees it has a small amount of coverage and in the third generation the application is at the initial stage.On the basis of a brief description of cDNA-AFLP technique in the basic principle and features,the application of cDNA-AFLP technique in fruit gene analysis was introduced especially,including the identification of bud mutation,gene isolation,gene expression analysis and construction of fingerprint.Finally,the prospect of cDNA-AFLP technique in the fruit tree biotechnology breeding was summarized.

关 键 词：CDNA-AFLP 基因鉴定 果树 

分 类 号：S66[农业科学—果树学]