PPARα和IL-1βmRNA在妊娠期子宫肌层的表达及相关性  

Expressions of peroxisome proliferator activated receptor alpha and interleukin-1 beta mRNA in human pregnant myometrium

作　　者：董坤[1] 张向丽[1] 苏雪莲[1] 刘媛[1] 

机构地区：[1]山东大学齐鲁医院妇产科,济南250012

出　　处：《山东大学学报（医学版）》2012年第9期105-108,123,共5页Journal of Shandong University:Health Sciences

摘　　要：目的探讨人类妊娠的不同阶段过氧化物酶体增殖物激活受体α(PPARα)和白细胞介素-1β(IL-1β)mRNA在子宫肌层的表达,以及在维持妊娠、发动分娩中的作用。方法收集10例未孕(NP组)、10例未足月未临产(PL组)、20例足月未临产(TNL组)及20例足月临产(TL组)育龄期妇女的子宫平滑肌组织,采用荧光定量(realtime)PCR技术检测PPARα及IL-1βmRNA的表达。以NP组作为对照,采用2-ΔΔCT法分析目的基因组间差异性表达。结果 PPARα在PL组的表达较其他各组均明显升高(P<0.05),在TL组的表达降低(P<0.05)。IL-1β在PL组的表达明显降低(P<0.05),在TL组的表达与其他各组比较则明显升高(P<0.05)。TNL组PPARα及IL-1β的表达与NP组比较差异均无统计学意义(P>0.05)。PPARα与IL-1β呈负相关(r=-0.767,P<0.05)。结论 PPARα及IL-1β在妊娠的不同时期均有明显变化。抗炎因子PPARα可能在维持妊娠中起重要作用,促炎因子IL-1β可能参与分娩发动。Objective To explore the expressions of peroxisome proliferator activated receptor alpha（PPARα）and interleukin-1 beta（IL-1β）mRNA in the human pregnant myometrium,and to investigate their roles in maintaining pregnancy or initiating labor.Methods Myometrium was obtained from women in the following four groups： non-pregnant（NP）（n=10）,preterm not in labor（PL）（n=10,gestation range 20-35 weeks）,term not in labor（TNL）（n=20,gestation range 37-41 weeks） and term in labor（TL）（n=20,gestation range 37-41weeks）.PPARα and IL-1β mRNA expressions were quantified by the real-time fluorescent quantitative PCR in the women myometrium.Non-pregnant myometrium was chosen as the control,and the changes and relationship of PPARα and IL-1β in different groups were analyzed by the 2-ΔΔCT relative quantification method.Results Compared to other groups,the expression level of PPARα in group PL was significantly higher（P0.05）,however the expression level in group TL was remarkably lower（P0.05）.The expression level of IL-1β in group PL was much lower than the other three groups（P0.05）,but the expression level in TL group obviously increased（P0.05）.There were no significant differences of the PPARα and IL-1β expressions between groups NP and TNL（P0.05）.PPARα expression was negatively correlated to the IL-1β expression（r=-0.767,P0.05）.Conclusion The expressions of PPARα and IL-1β obviously change in the human pregnant myometrium.Activation of PPARα may have a role to maintain pregnancy,and IL-1β may play a role in initiating labor.

关 键 词：妊娠 子宫肌层 过氧化物酶体增殖物激活受体Α 白细胞介素1Β 分娩发动 

分 类 号：R714.12[医药卫生—妇产科学]