染料介导光氧化及京尼平交联处理的同种异体动脉性能研究  

Research on the properties of allogenic arteries cross-linked by dye-mediated photooxidation and Genipin

作　　者：张冬[1] 张军[1] 刘鲁祁[1] 

机构地区：[1]山东大学附属千佛山医院心脏外科,济南250014

出　　处：《山东大学学报（医学版）》2012年第10期41-45,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(Y2007C011)

摘　　要：目的利用不同交联方法处理的生物组织具有不同生物学特性,观察染料介导光氧化(DMP)及京尼平(GP)交联后的同种异体动脉的物理特性及生物相容性。方法以新西兰兔胸主动脉(血管内径4 mm左右)为研究对象,按对其交联处理方法的不同分为DMP交联处理组(DMP组)、GP交联处理组(GP组)、戊二醛(GA)交联处理组(GA组),同时设立实验对照组(即未进行任何特殊交联处理)。经不同交联处理后,测定各组的组织含水量、热皱缩温度、断裂强度,并进行体外溶血试验,观察处理后动脉的生物相容性。结果形态学观察:GP组与GA组透光度减低,弹性、韧性明显增强,DMP组透光度较高,弹性、韧性未见增强;组织含水量:对照组>DMP组>GP组>GA组;热皱缩温度:GA组>GP组>DMP组>对照组;断裂强度:GA组>GP组>DMP组>对照组;体外溶血指数:GA组>GP组>DMP组。结论染料介导光氧化及京尼平交联处理的血管材料显示出良好的物理特性和生物相容性,适宜塑型及体内植入。Objective To detect the physical characteristics and the biocompatibility of allogenic arteries cross-linked by dye-mediated photooxidation （DMP） and Genipin （GP） based on the former researches that biological tissue which have gone through different cross-linked methods will show up different biological properties. Methods The thoracic arteries of New Zealand rabbits （with the inside vessel diameter of 4mm）, according to different cross-linked methods, were divided into the DMP group, the GP group, the Glutaraldehyde （GA） group, and a control group was also estab- lished （which had not gone through any cross-linked treatment）. After the arteries were cross-linked with different methods, tissue water content, heat shrinking temperature, breaking tenacity, and biocompatibility which had gone through the external hemolytic test were detected. Results Morphological observation： The transmittance of the GP group and the GA group was reduced, while the elasticity and toughness were obviously enhanced. The transmittance of the DMP group was superior, while the elasticity and toughness were not enhanced; Tissue water content： control group 〉 DMP group 〉 GP group 〉 GA group; Heat shrinking temperature： GA group 〉 GP group 〉 DMP group 〉 control group; Breaking tenacity： GA group 〉 GP group 〉 DMP group 〉 control group; Indices of external hemolytic： GA group 〉 GP group 〉 DMP group. Conclusions Vascular materials cross-linked by dye-mediated photooxidation and Genipin have shown better physical characteristics and biocompatibility. So it is suitable for molding and implanting in human body.

关 键 词：染料介导光氧化DMP 京尼平GP 同种异体动脉 交联处理 

分 类 号：R654.3[医药卫生—外科学]