SOX4基因在前列腺癌中的表达及二烯丙三硫对其表达的调节作用  

Expression of SOX4 and its regulation by diallyl trisulfide in prostate cancer

作　　者：张晶[1] 王林[2] 杨晓庆[2] 王春妮[2] 王妍[3] 张娟[3] 韩博[2,3] 

机构地区：[1]山东大学附属省立医院药剂科,济南250021 [2]山东大学医学院病理学教研室 [3]山东大学齐鲁医院病理科,济南250012

出　　处：《山东大学学报（医学版）》2012年第10期100-105,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81072110);山东大学自主创新基金(2010TB012)

摘　　要：目的研究SOX4基因在前列腺癌患者中的表达;探讨二烯丙三硫(DATS)对SOX4表达阳性前列腺癌细胞的生物学作用及对SOX4表达的调节作用。方法采用免疫组织化学PV-9000二步法检测141例前列腺癌患者SOX4的表达,观察其与临床病理指标和预后的关联。采用不同浓度(0、20、40、60μmol/L)DATS处理前列腺癌Vcap细胞株,细胞增殖活性MTS法测定Vcap细胞的增殖能力;细胞体外侵袭实验检测40μmol/L DATS对Vcap细胞侵袭能力的作用;实时定量PCR(Real-time PCR)检测40μmol/L DATS作用后,Vcap细胞中SOX4mRNA的表达水平变化。结果 SOX4蛋白在21%(28/135)的前列腺癌患者中过表达,与Gleason评分、远处转移呈正相关(P<0.05)。SOX4是影响患者生存的独立预后因素。体外实验显示,20～60μmol/L DATS对前列腺癌细胞株Vcap的生长有较明显的抑制作用,且呈浓度和时间依赖性。40μmol/L DATS明显抑制Vcap细胞的侵袭能力,并在mRNA水平上下调SOX4的表达。结论 SOX4的过表达提示前列腺癌患者预后不良。DATS抑制前列腺癌Vcap细胞的增殖和侵袭能力,并在mRNA水平上显著下调SOX4的表达。Objective To characterize the expression of SOX4 in prostate cancer patients and investigate the effect of di- allyl trisulfide （DATS） on proliferation and invasion of SOX4-positive prostate cancer cells and its role in regulation of SOX4. Methods The PV9000 two-step immunohistochemical method was utilized to examine the expression of SOX4 protein in 141 prostate cancer patients. Correlation of SOX4 expression with clinicopathological parameters and progno- sis were also analyzed. Prostate cancer cell line Vcap, which demonstrated overexpression of SOX4, was treated with various concentrations of DATS （0,20,40,60 μmol/L）, followed by MTS assay to determine its cellular proliferation; Matrigel invasion assay was used to assess the effect of 40 p.mol/L DATS on the invasive capacity of Vcap cells. Real time-PCR was performed to detect mRNA expression level of SOX4 by 40μmol/L DATS treatment. Results Overex- pression of SOX4 protein was preset in 21% （28/135） of prostate cancer patients, and significantly correlated with high Gleason score and the presence of distant metastasis （ P 〈 0.05 ）. SOX4 was an independent prognostic factor for prostate

关 键 词：SOX4基因 二烯丙三硫 前列腺肿瘤 预后 侵袭 

分 类 号：R966[医药卫生—药理学] R979.1[医药卫生—药学]