HPLC法测定追风透骨片中龙胆苦苷的含量  

Determination of Gentiopicroside in Zhuifeng Tougu Tablets by HPLC

作　　者：任春[1] 武洋[1] 王怡君[1] 

机构地区：[1]辽宁省食品药品检验所,沈阳110023

出　　处：《中国药事》2012年第9期1003-1005,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立HPLC法测定追风透骨片中龙胆苦苷的含量。方法采用迪马Diamonsil C18色谱柱;流动相为甲醇-水(20∶80),检测波长为254nm。结果龙胆苦苷进样量在0.02～1.01μg范围内线性关系良好(r=0.9993);平均回收率为100.6%,RSD为0.96%(n=6)。结论本法简单、准确,可用于追风透骨片中龙胆苦苷的含量测定。Objective To establish an HPLC method for determination of gentiop Tougu Tablets. Methods A Diamonsil C18 column was used with methanol-water （ phase. The detection wavelength was set at 254 nm. Results The linear range was 0. 9993）. The average recovery was 100.6% and RSD was 0.96% （n=6）. Conclusi simple and accurate. It could be used for determination of gentiopicroside in Zhuifeng Tougu Tablets.

关 键 词：追风透骨片 含量测定 高效液相色谱法 龙胆苦苷 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]