十种热带雨林植物光活化抗微生物活性的筛选  

Photo-Activitied Antimicrobial Activities Screening of 10 Tropical Rain Forest Plants

作　　者：胡昀[1] 陈玉[2] 

机构地区：[1]中南民族大学药学院,武汉430074 [2]中南民族大学化学与材料科学学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2012年第3期63-65,100,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(30670215)

摘　　要：为从植物中寻找具有光调节生物活性的物质,通过滤纸片法和薄层色谱自显影技术,以金黄色葡菌球菌(Staphylococcus aureus)、枯草芽胞杆菌(Bacillus subtilis)、草分枝杆菌(Mycobacteium phlei)、环状芽胞杆菌(Bacillus circulans)为供试菌,对10种热带雨林植物进行光活化抗微生物活性的筛选和活性部位的确定.结果表明:0.1mg的狗花椒(Y10)氯仿提取物对枯草芽胞杆菌具有明显的光活化抗微生物活性,在紫外光照射后和黑暗中抑菌圈相差4mm,薄层色谱中0.5mg Y10氯仿提取物抗枯草芽胞杆菌的抑菌圈最大.本研究为进一步从该植物中寻找新型光敏剂奠定了基础.To find the light-medidated biological active substances, 10 tropical rain forest plants were screened by the paper disc method and thin-layer chromatography bioautography technique to test their antimicrobial activity Staphylococcus aureus,Bacillus subtilis, Mycobacteium phlei and Bacillus circulan were employed as samples. The results showed that 0. ling of Zanthoxylum planispinum sieb. et Zucc. （Y10） chloroform extracts have obvious photo-aetivitied antimicrobial activities for Bacillus subtilis. The antibacterial circle difference between the uhra-violet light and darkness is 4mm. And 0.5rag Y10 chloroform extracts have maximum inhibition zone for Bacillus subtilis in thin-layer chromatography. Our study can be used for further investigation in novel photosensitizers.

关 键 词：光活化抗微生物活性 滤纸片法 薄层色谱自显影技术 

分 类 号：Q939.92[生物学—微生物学]