原花青素对脊髓损伤大鼠运动功能的影响  

Effect of Proanthocyanidin on Motor after Spinal Cord Injury in Rats

作　　者：谢少华[1] 杨拯[1] 龚都[1] 张尧[1] 余婷[1] 高建华[1] 杨海燕[1] 李晓[1] 马延仃[1] 张晓[1] 

机构地区：[1]成都医学院,四川成都市610081

出　　处：《中国康复理论与实践》2012年第9期831-833,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：成都医学院创新性实验项目资助(CX201219)

摘　　要：目的观察原花青素对脊髓损伤大鼠运动功能恢复的影响。方法健康成年SD大鼠36只,采用Allen法(250 g.mm)在T9复制急性脊髓损伤模型,分为A、B、C 3组,每组12只。造模型成功后30 min,A组腹腔注射原花青素40 mg/kg,B组腹腔注射甲基强的松龙30 mg/kg,C组腹腔注射等体积生理盐水。于术后1 d、3 d、7 d对各组进行后肢功能BBB评分、斜板试验,血清丙二醛(MDA)和超氧化物歧化酶(SOD)测定。结果 BBB评分和斜板试验结果均显示,术后3 d和7 d,A、B两组较C组后肢运动功能恢复更好(P<0.05)。与C组相比,A、B两组术后1 d和3 d SOD活性升高,MDA含量降低(均P<0.05);7 d时,A组较C组SOD活性增高,MDA降低(P<0.05)。结论原花青素可有效抑制脂质过氧化反应,对脊髓损伤大鼠运动功能的恢复具有促进作用。Objective To observe the effect of Proanthocyanidin on motor after spinal cord injury（SCI） in rats.Methods 36 healthy adult SD rats were divided into groups A,B and C（n=12）,and SCI was induced with Allen＇s mode（250 g·mm） on T9.Proanthocyanidin 40 mg/kg was injected intraperitoneally for group A,methylprednisolone（MP） 30 mg/kg for group B and the same volume of saline for group C 30 min after SCI.1 d,3 d and 7 d after operation,all the rats were assessed with Basso-Beattie-Bresnahan（BBB） scale and slanting board test,and their serumal malondialdehyde（MDA） and superoxide dismutase（SOD） were detected.Results The scores of BBB and the slanting board test imporoved more in group A and group B than in group C（P〈0.05）.The SOD increased and MDA decreased in groups A and B significantly 1 d and 3 d after operation compared with those of group C（P〈0.05）,and only in group A 7 d after operation（P〈0.05）.Conclusion Proanthocyanidin may inhibit the lipid peroxidation and promote the recovery of motor after spinal cord injury in rats.

关 键 词：原花青素 脊髓损伤 运动功能 超氧化物歧化酶 丙二醛 

分 类 号：R651.2[医药卫生—外科学]