南方红豆杉愈伤组织诱导初探  

The Induction of Callus Tissue of Taxus chinensis var.mairei

作　　者：于娅[1] 陈嘉棋[1] 

机构地区：[1]武汉生物工程学院生物工程系,武汉430415

出　　处：《中国农学通报》2012年第28期39-43,共5页Chinese Agricultural Science Bulletin

基　　金：湖北省教育厅指导项目(B20104602)

摘　　要：为了研究南方红豆杉愈伤组织诱导的影响因素,选取不同外植体、不同外源激素、不同光照条件进行研究。结果表明:(1)茎段是诱导红豆杉愈伤组织的最佳外植体,出愈时间快,愈伤组织黄绿色,质地疏松;而叶片则出愈时间慢,愈伤组织黄白色,质地紧密。(2)2,4-D与NAA的协同促进作用明显,当2,4-D/NAA为1/5时,诱导效果最好,而比值较大时不利于愈伤的诱导。(3)黑暗培养能够明显促进愈伤组织发生,褐化现象减轻。In order to investigate the factors influencing the induction of callus tissue from Taxus chinensis var. mairei, the author researched on callus induction from stem and needle leaves. The results indicated that： young stem was the best explant of the callus induction, callus tissue grew fast and appeared yellow green with loose texture, while the callus tissue from leaves were white and slow in growth. The combination of 2,4-D and NAA could promote the induction of callus tissue, and 1/5 of 2,4-D/NAA was the best. Culture in darkness could significantly benefit the callus tissue and reduce the browning phenomenon.

关 键 词：南方红豆杉 愈伤组织 外植体 褐化 

分 类 号：Q813.12[生物学—生物工程]