艾灸对大鼠应激性胃黏膜损伤修复及p-ERK1/2蛋白表达的影响  

Moxibustion promoting repair of gastric mucosa in rats with stress-induced gastric mucosal injury and up-regulating the expression of p-ERK1/2

作　　者：刘密[1] 常小荣[1] 易受乡 严洁 林亚平 张泓[1] 岳增辉[3] 彭艳[3] 

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410208 [2]国家中医药管理局经穴-脏腑相关重点研究室,长沙410007 [3]湖南省教育厅针灸生物信息分析重点实验室,长沙410007

出　　处：《中华中医药杂志》2012年第10期2640-2643,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)(No.2009CB522904);国家自然科学基金项目(No.81173326;No.81202770);湖南省高校创新平台开放基金项目(No.11K047);湖南省研究生科研创新项目资助(No.CX2009B172)~~

摘　　要：目的:探讨艾灸促进大鼠应激性胃黏膜损伤修复作用及细胞机制。方法:SD大鼠48只随机分为空白组、模型组、艾灸穴位组、艾灸非穴组,每组12只。束缚冷应激法制作应激性胃黏膜损伤大鼠模型,在造模之前,艾灸穴位组选取足三里、中脘、脾俞、胃俞等穴位行艾灸预处理8d。按Guth法计算胃黏膜损伤指数(UI),免疫组化、蛋白质印迹法(Western Blot)检测胃黏膜磷酸化细胞外信号调节激酶1/2(phosphorylating extracellularsignal-regulated kinase1/2,p-ERK1/2)蛋白表达。结果:与空白组比较,模型组大鼠UI值显著增高(P<0.01)、胃黏膜细胞p-ERK1/2蛋白表达加强(P<0.05,P<0.01);与模型组、艾灸非穴组比较,艾灸穴位组大鼠UI值明显下降(P<0.01)、胃黏膜细胞p-ERK1/2蛋白表达增强(P<0.01)。结论:艾灸足三里、中脘等穴位预处理可促进束缚水浸应激所造成大鼠胃黏膜损伤的修复,其作用机制可能与上调胃黏膜p-ERK1/2蛋白表达有关。To explore the effect and cellular mechanism of moxibustion on stress-induced gastric mucosal injury in rats.Methods： 48 SD rats were randomly allocated to blank,model,acupoints moxibustion（AM） and non-acupoints moxibustion（N-AM） groups.The gastric mucosal lesions model was developed by restraint and water immersion stress.Pre-moxibustion was applied to ＇Zusanli＇（ST 36） and ＇Zhongwan＇（RN 12）,＇Pishu＇（BL 20） and ＇Weishu＇（BL 21） alternately once everyday for 8 days before modeled.The ulcer index（UI） was evaluated by Guth method,the expressions of p-ERK1/2（phosphorylating extracellular signal-regulated kinase1/2） determined by immunohistochemistry and western blot assay.Results： Compared with blank group,UI index and the expressions of p-ERK1/2 increased significantly（P0.01,P0.05） in model group.Compared with model and N-AM groups,UI index decreased markedly（P0.01） and the expressions of p-ERK1/2 increased significantly（P0.01） in AM group.Conclusion： Pre-moxibustion at ST 36,RN 12,BL 20 and BL 21 could up-regulate p-ERK1/2 protein expression in gastric mucosa of rats with gastric mucosal injury,which possibly contributed to its effect in promoting repair of gastric mucosa.

关 键 词：艾灸 应激性胃黏膜损伤 细胞外信号调节激酶 

分 类 号：R245[医药卫生—针灸推拿学]