针刺对局灶性脑缺血大鼠凝血-纤溶系统的影响  

Effects of acupuncture on coagulation-fibrinolysis system in rats with focal cerebral ischemia

作　　者：孙琳琳[1] 文娜 图娅[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029 [2]煤炭总医院,北京100028

出　　处：《中华中医药杂志》2012年第10期2647-2650,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家科技重大专项"重大新药创制"课题(No.2009ZX09103-707)~~

摘　　要：目的:观察针刺对局灶性脑缺血大鼠凝血-纤溶系统的影响。方法:将雄性Wistar大鼠随机分为空白对照组,模型对照组,针刺治疗组与尼莫地平组,以局灶性脑缺血模型大鼠为实验对象,观察凝血时间(CT)、凝血酶时间(TT)、凝血酶原时间(PT)、部分凝血活酶时间(APTT)、优球蛋白降解时间(ELT),ELISA法检测血浆组织型纤溶酶原激活物(t-PA)、纤溶酶原激活物抑制剂(PAI)表达变化,并对结果进行统计分析。结果:与模型对照组比较,针刺后CT显著性延长(P<0.01),TT、PT、APTT值显著延长(P<0.05),ELT值显著性缩短(P<0.01),t-PA活性显著上升(P<0.05),PAI活性显著下降(P<0.05),且针刺治疗组的CT明显长于尼莫地平组(P<0.05),ELT值显著小于尼莫地平组(P<0.05)。结论:针刺可能通过改善局灶性缺血大鼠凝血-纤溶系统异常,发挥神经保护与抗栓溶栓的作用。Objective： To observe the influence of acupuncture on coagulation-fibrinolysis system in rats with focal cerebral ischemia. Methods： Rats were divided into four groups randomly： blank group, model group, acupuncture group and Nimodipine group, we established the focal cerebral ischemia model, to observe the coagulation time（CT）, to detect the thrombin time（TT）, prolonged time（PT）, activated partial thromboplasfin time（AFIT）, and euglobulin lysis time（ELT）. Tissue- type plasminogen activator（t-PA） and plasminogen activator inhibitor（PAI） were tested by ELISA. Result： Compared with the model group, obviously prolonged effect in CT （P〈0.01）, obviously prolonged effectin TT, PT, APTT（P〈0.05）, obviously shortening effect in ELT were found in the acupuncture group（P〈0.01）, also acupuncture could significantly improve the activity of t-PA（P〈0.05） and lower the activity of PAI（P〈0.05）, and there were statistical significance between acupuncture group and Nimodipine group in CT（P〈0.05） and ELT（P〈0.05）. Conclusion： Improving the coagulation-fibrinolysis system＇s function may be one of the ways of acupuncture＇s.

关 键 词：针刺 局灶性脑缺血 凝血-纤溶系统 凝血时间 优球蛋白降解时间 

分 类 号：R245[医药卫生—针灸推拿学]