黄芪有效部位对糖尿病大鼠Na^+-K^+-ATP酶活性及AMPK蛋白表达的影响  

Effects of astragalus active ingredients on Na^+-K^+-ATPase and AMP-activated protein kinase protein expression in diabetic rats

作　　者：范颖[1] 李楠[1] 孙云峰[1] 马哲[1] 林庶茹[1] 

机构地区：[1]辽宁中医药大学,沈阳110847

出　　处：《中华中医药杂志》2012年第10期2660-2663,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30873232);辽宁省优秀人才项目(No.2009R39)~~

摘　　要：目的:通过研究糖尿病模型大鼠胰腺组织钠-钾-ATP酶(Na+-K+-ATP)活性、腺苷酸激活蛋白激酶(AMPK)活性及肝、骨骼肌组织AMPK蛋白表达的变化,探讨黄芪有效部位对糖尿病大鼠能量代谢的影响。方法:SD大鼠随机分为正常组、模型组、中药对照组、黄芪组、黄酮组、多糖组、皂苷组、酮糖组、酮苷组、糖苷组、酮糖苷组,共11组,每组14只。由链脲佐菌素(52mg/kg)诱导糖尿病大鼠模型,造模同日给予黄芪及其有效部位进行干预。观测30日,检测血糖,生化法分析胰腺组织Na+-K+-ATP酶的活性,ELISA法分析胰腺AMPK活性,Western Blot法分析肝、骨骼肌组织AMPK蛋白表达。结果:糖尿病模型大鼠血糖显著升高(P<0.01),胰腺Na+-K+-ATP酶活性、AMPK水平以及肝、骨骼肌AMPK蛋白表达均显著降低(P<0.01);与模型组比较,黄芪组、黄酮组、酮糖组、酮糖苷组血糖降低(P<0.05,P<0.01),胰腺Na+-K+-ATP酶活性、AMPK水平显著升高(P<0.01),酮苷组血糖下降、胰腺Na+-K+-ATP酶活性升高(P<0.01),皂苷组、糖苷组胰腺Na+-K+-ATP酶活性升高(P<0.01)。结论:黄芪、黄芪黄酮及含黄芪黄酮的有效部位能够降低糖尿病大鼠的血糖,其机制可能与改善Na+-K+-ATP酶活性、上调AMPK水平及蛋白表达有关。Objective： This study is designed to evaluate the effect of astragalus active ingredients on energy metabolism in diabetic rats by studying Na^＋-K^＋-ATPase and AMPK in pancreas and AMPK protein expression in liver and skeletal muscle. Methods： Diabetes rats were induced by STZ （52mg/kg, peritioneal injection）. Diabetic rats were administered by Astragalux radix and its active ingredients for 30 days from the day of STZ pj. Astragalus radix active ingredients were astragalux radix group （AS）, astragalus flavonoids group （ASF）, astragalus polysaccharides group （ASP）, and astragalosides group （ASS）, astragalus flavonolids and polysaccharides group （ASF＋ASP）, astragalux flavonolids and astragalosides group （ASF＋ASS）, astragalux polysaccharides and astragalosides group （ASP＋ASS）, astragalus flavonolids and polysaccharides and astragalosides group （ASF＋ASP＋ASS）. Then blood glucose was detected, biochemical analysis of Na^＋-K^＋-ATPase in pancreas, EL1SA assay of pancreas AMPK also were administrated, AMPK protein expression by western blotting in liver and skeletal muscle at 30th day. Results： The blood glucose of diabetes rats ascended （P〈0.01）, Na^＋-K^＋-ATPase and AMPK descended in pancreas of diabetic rats, AMPK protein expression also descended in liver and skeletal of diabetic rats （P〈0.01）. AS, ASF, ASF＋ASP, ASF＋ASP＋ASS all decreased blood glucose （P〈0.05, P〈0.01 ）, ascended Na^＋-K^＋-ATPase and AMPK in pancreas. ASF＋ASS decreased blood glucose and elevated Na^＋-K^＋-ATPase in pancreas （P〈0.01）, ASS, ASP＋ASS also elevated Na^＋-K^＋-ATPase in pancreas （P〈0.01）. Conclusion： Astragalus Radix and astragalus flavonoids could decrease blood glucose, it may be related to regulating Na^＋-K^＋-ATPase and AMPK in pancreas and AMPK protein expression in liver and skeletal muscle.

关 键 词：黄芪 黄芪有效部位 糖尿病 钠-钾-ATP酶 腺苷酸激活蛋白激酶 

分 类 号：R285.5[医药卫生—中药学]