复方中药对代谢综合征SD大鼠的干预研究  

Study of intervention with compound TCM in metabolic syndrome rats

作　　者：夏瑢[1] 关昊[2] 

机构地区：[1]浙江中医药大学基础医学院,杭州310053 [2]浙江中医药大学第二临床医学院,杭州310005

出　　处：《中华中医药杂志》2012年第10期2668-2671,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省科技厅科研专项任务资助(No.2006C30043)~~

摘　　要：目的:观察益气活血化浊中药对代谢综合征(MS)模型大鼠的干预作用。方法:雄性SD大鼠60只,高脂高盐饲料喂养复制MS大鼠模型。随机分为正常组、模型组、文迪雅组、及中药(低、中、高剂量)组,中药组予中药、正常组和模型组予等体积0.9%氯化钠溶液灌胃;治疗4周后测血糖、胰岛素、血脂、瘦素、胰岛素敏感指数(ISI)、脂肪组织过氧化物增殖体激活受体(PPARγmRNA)表达。结果:①中药各组体质量、内脏脂肪重量及系数、瘦素水平均显著低于模型组(P<0.05,P<0.01),但文迪雅组降低无统计学意义;②中药中剂量和文迪雅组的餐后2h血糖、血脂均显著性低于模型组而ISI显著高于模型组(P<0.05);③中药中剂量组PPARγmRNA表达比值显著高于模型组(P<0.05),但低于文迪雅组(P<0.01)。结论:该复方中药促进MS大鼠脂肪组织PPARγmRNA表达作用虽弱于文迪雅,但兼有纠正糖脂代谢紊乱、减肥、改善胰岛素、瘦素抵抗等多重作用。Objective： To observe the infulence of compound TCM with strengthening Qi, promoting blood circulation and discharging trash function on SD rats with metabolic syndrome（MS）. Methods： Fed 60 male SD rats with high fat-salt diet to establish a model which resembled human character of MS. Rats were randomly divided into groups： normal group, model group, rosiglitazone group, low-dose, middle-dose and high-dose TCM groups. The TCM groups were administered intragastrically with compound TCM, normal and model group with equal volume normal saline. Administered for 4 weeks, tested blood glucose, insulin, serum lipid and leptin, detected PPAR γ expression of omental grease by RT-PCR. Results： ①In compound TCM groups, body weight, weight and coefficient of visceral fat were significantly lower than those in model group（P〈0.05, P〈0.01） and the decrease in rosiglitazone group had no statistical significance. ②In rosiglitazone and middle-dose TCM group, the blood glucose and serum lipid were significantly lower and ISI was higher than those in model group（P〈0.05）. ③In middle-dose TCM group, expression of PPAR γ mRNA was significantly higher（P〈0.05） than in model group, was lower than in rosiglitazone group（P〈0.01）. Conclusion： These indicate that the compound TCM with strengthening Qi, promoting blood circulation and discharging trash function has an effect on promoting omental grease PPAR γ mRNA expression, which is weaker than rosiglitazone and has combining multiple effects of enhancing insulin sensitivity, retrieving sugar-lipid metabolic disorder, decreasing body weight and improving leptin resistance.

关 键 词：益气活血化浊中药 代谢综合征 PPARΓ mRNA 瘦素 

分 类 号：R285.5[医药卫生—中药学]