蕲蛇水提液对佐剂性关节炎大鼠的免疫调节作用  

Immunomodulatory effects of aqueous extract of Agkistrodon acutus on adjuvant arthritis rats

作　　者：谷恒存[1] 丁兴红[1] 马哲龙[1] 温成平[1] 丁志山[1] 陈建真[1] 范永升[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《中华中医药杂志》2012年第10期2676-2678,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省重点科技创新团队项目(No.2011R09042-03;2011R09042-04)~~

摘　　要：目的:研究蕲蛇水提液对佐剂性关节炎(AA)模型大鼠的治疗作用,并探讨其作用机制。方法:制备完全弗氏佐剂诱导的Wistar大鼠AA模型并进行随机性分组,分为空白组、模型组、阳性组(塞来昔布给药组)、蕲蛇低剂量组、蕲蛇中剂量组、蕲蛇高剂量组,每组10只。连续给药20d,采用容积法测量关节肿胀程度,ELISA法检测血清肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)的表达水平。结果:与模型组比较,不同剂量的蕲蛇水提液均能显著降低大鼠右后足跖肿胀度(P<0.01),使炎性细胞因子TNF-α、IL-1β、IL-6水平均显著降低(P<0.01),且对不同炎性细胞因子的抑制强度与阳性组相比有所不同。结论:蕲蛇提取液对AA大鼠关节炎症有一定的治疗作用,其治疗机制可能是通过口服免疫耐受机制产生免疫调节作用抑制炎性细胞因子实现的。Objective： To explore the therapeutic effects and mechanisms of the aqueous extract of Agkistrodon acutus on adjuvant arthritis （AA） in rats. Methods： The Wistar rats were divided into normal, model, celecoxib and treatment group after modeled by Freund＇s complete adjuvant （FCA）. The paw volume was measured to determine joint swelling degree of the AA rats. After treated the model rats for 20 days collected the blood of all rats to measure the level of TNF-α, IL-1β and IL-6,determined by Enzyme-linked Immunosorbent Assay （ELISA） kits. Results： The aqueous extract of Agkistrodon acutus could decrease significantly the paw swelling degree （P〈0.01）, decrease the level of TNF-α, IL-1β and IL-6 protein in the AA rats serum （P〈0.01）. For different cytokines, treatment group have different inhibition effects compared to celecoxib group. Conclusion： The aqueous extract of Agkistrodon acutus could inhibit the inflammation of the AA rats. Mechanisms underlying it may be through oral tolerance to achieve the purpose of reduce the expression of TNF- α, IL-1β and IL-6.

关 键 词：蕲蛇 佐剂性关节炎 类风湿关节炎 口服免疫耐受 肿瘤坏死因子-α 白介素-1Β 白介素-6 

分 类 号：R285.5[医药卫生—中药学]