基于蛋白芯片技术研究参苈加颗粒对慢性心衰大鼠心肌MCP-1表达的影响  

Effects of Shenlijia Granule on MCP-1 expression in heart failure rats screened by the protein chips

作　　者：陈飞[1] 解颖[1] 隋博文[1] 高恩宇[1] 李富震[1] 乔羽[1] 王兵[1] 白玉宾[1] 姜德友[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中华中医药杂志》2012年第10期2702-2704,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：哈尔滨市科技局科技创新人才研究专项(No.RC2009XK004075)~~

摘　　要：目的:应用蛋白芯片技术筛选并分析参苈加颗粒对心力衰竭大鼠心肌单核细胞趋化蛋白-1(MCP-1)表达的影响。方法:将70只清洁级Wistar大鼠随机抽取空白组15只,其余用阿霉素造模,将成模大鼠随机分为模型组和治疗组,分别给予参苈加颗粒和0.9%氯化钠溶液灌胃,每日1次。4周后取大鼠左心室组织。结果:空白组心肌正常;模型组心肌纤维增粗、紊乱,间质增生,少量炎症细胞浸润;治疗组心肌排列较整齐,间质无明显增生,未见炎症细胞浸润。蛋白芯片显示,与空白组相比,模型组的表达下调;与模型组比较,治疗组的表达上调;治疗组与空白组比较无表达差异。结论:参苈加颗粒能够通过上调MCP-1的表达,对心力衰竭大鼠的心肌起到保护作用。Objective： To assess the effect of Shenlijia Granule on MCP-1 expression in heart failure rats by the protein chips. Methods： Total of seventy Wistar rat were randomly selected 15 to divide into blank group, the others were induced by intraperitoneal injection of doxorubicin. The survival rats were randomly divided into model and treatment group. After the model establishment, the rats had been fed for 28 days. Meanwhile, rats in the treatment group were treated with gastric perfusion of Chinese herb Shenlijia Granule, once a day for twenty days. And those in the blank and modle group were treated with gastric perfusion saline. Four weeks later, gathering the left cardiac muscular tissue,the apical tissue was soaked in 4% paraformaldehyde for HE stain, the left was kept in liquid nitrogen for the protein chips. Results： HE stain display that the blank group was normal; the myocardium of the model group was coarse, interstitial proliferation and inflammatory cells infiltration. The treatment group had no obvious and inflammatory cells infiltration. The protein chips indicated that MCP-1 was differentiated. Compared with blank group, the model of expression was decreased; the treatment of expression was increased; there was no difference between the group treatment and blank. Conclusion： Shenlijia Granule is able to protected the rat insufficiency heart by upregulating the expressive of MCP- 1.

关 键 词：蛋白芯片 参苈加颗粒 心力衰竭 单核细胞趋化蛋白-1 

分 类 号：R285.5[医药卫生—中药学]