寒痹康汤对实验性关节炎大鼠滑膜细胞凋亡的影响  

The effect of HanBiKang Decoction on apoptosis of synovial cell in CIA rats

作　　者：庞学丰[1] 吴燕红[1] 姚平[1] 李爱媛[1] 石宏斌[1] 赖申昌[1] 巩志富[1] 卢阳佳[1] 龙朝阳[1] 潘燕[1] 王乾[1] 

机构地区：[1]广西中医药大学附属瑞康医院,广西南宁530011

出　　处：《风湿病与关节炎》2012年第1期42-45,共4页Rheumatism and Arthritis

基　　金：广西科技厅科研项目(桂科青0640057)

摘　　要：目的:观察寒痹康汤对实验性关节炎大鼠滑膜细胞凋亡的影响,从细胞凋亡的角度探讨寒痹康汤治疗类风湿关节炎的作用机理。方法:采用牛II型胶原蛋白诱导实验性关节炎模型,以甲氨蝶呤作对照,灌服寒痹康汤进行治疗,取膝关节滑膜组织,一部分经固定、脱钙、包埋、切片;另一部分用胶原酶消化法进行原代培养,取3～5代的传代细胞,进行实验观察。分别采用原位细胞凋亡检测法(TUNEL法)、流式细胞仪检测各组大鼠滑膜细胞凋亡情况。结果:寒痹康汤各治疗组滑膜细胞凋亡均较模型组增加(P<0.05或P<0.01),其中高剂量组与甲氨蝶呤比较有显著性差异(P<0.05)。结论:寒痹康汤能诱导实验性关节炎大鼠滑膜细胞凋亡,是其治疗类风湿关节炎的作用机理之一。Objective：To observe the effect of HanBiKang Decoction on apoptosis of synovial cell in CIA rats, and to explore the treatment mechanism of HanBiKang Decoction on CIA rats. Methods ：The model of experimental arthritis of rats established by Bovine collagen type Ⅱ was divided into the treatment groups and the control group. The rats of the control group was taken the methotrexate while the rats of the treatment groups was taken the different doses of HanBiKang Decoction. Collect synovial tissues in knee joint of each rat. Each synovial tissues was divided into two parts, one was fixed, decalcified, embedded in paraffin, sec- tioned and detected by terminal deoxynucleotidy transferase-mediated dUTP nick-end labeling（ TUNEL）. The other was used for primary culture by collagenase digestion method and the cells of passage3 -5was performed to analyze cell apoptosis by flow cytometry. Results： Compared with model group, the apoptosis of synovial cell in knee joint of CIA rats in control group and treatment groups increase significantly（ P 〈 0.05orP 〈 0.01 ）. And there is a significant difference between the control group and the highdosage HanBiKang Decoction group. Conclusion ： One of the the treatment mechanism of HanBiKang Decoction on rheumatoid arthritis is in- creasing synovial cell apoptosis.

关 键 词：关节炎 类风湿 滑膜 寒痹康汤 动物实验 

分 类 号：R285.5[医药卫生—中药学]