15个VNTR位点用于87株丽水市结核分枝杆菌分离株的基因分型研究  

Study on the genotyping of 87 Mycobacterium tuberculosis isolates in Lishui based on 15 variable number of tandern DNA repeats

作　　者：雷永良[1] 陈秀英[1] 梅建华[1] 陶毅[1] 兰进权[1] 王晓光[1] 

机构地区：[1]浙江省丽水市疾病预防控制中心,浙江丽水323000

出　　处：《中国卫生检验杂志》2012年第9期2011-2013,2017,共4页Chinese Journal of Health Laboratory Technology

基　　金：丽水市科技计划资助项目(丽科2007-70)

摘　　要：目的:选取2008年-2010年间丽水市结核分枝杆菌临床分离株,通过多位点可变数目串联重复序列分型,了解丽水市结核分枝杆菌流行菌株基因分型情况。方法:对临床分离株进行常规培养,收集菌体,提取基因组DNA,采用聚合酶链反应(PCR)分别对15个VNTR位点进行检测分析,基因聚类分析采用BioN-umerics软件。结果:经聚类分析,87株结核分枝杆菌分为4个基因群(I群、Ⅱ群、Ⅲ群、Ⅳ群)69个基因型。其中Ⅳ群占74.7%,含49个基因型,I群占9.2%,含8个基因型,Ⅱ群占8%,含7个基因型,Ⅲ群占5.75%。含5个基因型。结论:丽水市的结核分枝杆菌存在基因多态性,其主要流行型为Ⅳ型。Objective：To analyze the genotype of epidemic Mycobacterium tuberculosis strains by multiple-locus variable number tandem repeat analysis（MLVA） in Lishui from 2008 to 2010.Methods： M.tuberculosis were cultured with Lowenstein-Jensen medium.The bacteria genome DNA was collected.The polymorphism of VNTR locus was tested in the DNA samples with PCR respectively.The clustering of genotype was analyzed with BioNumerics.Results： The results showed that there were obvious polymorphisms of VNTRs.The clustering of genotype showed that these strains could be categorized into 4 gene clusters（I,Ⅱ,Ⅲ,Ⅳ） and 69 genotypes.74.7% were cluster Ⅳ including 49 genotypes,9.2% were cluster I including 8 genotypes,8% were clusterⅡ including 7 genotypes,5.75% were cluster Ⅲ including 5 genotypes.Conclusion： There were obvious polymorphisms of VNTRs of M.tuberculosis cilinical strains preliminarily.There were at least 4 clusters of VNTR genotype and cluster Ⅳ was the predominant prevalent strain in Lishui city.

关 键 词：结核分枝杆菌 基因分型 聚类分析 数目可变串联重复序列技术 

分 类 号：R378.911[医药卫生—病原生物学]