三种梅毒血清学检测方法的适应性分析  

A study on the stability of 3 syphilis serological surveys

作　　者：虞静[1] 蒋逸群[1] 凌丰[1] 周惠芳[1] 钱建新 

机构地区：[1]浙江省海宁市疾病预防控制中心,浙江海宁314400 [2]浙江省桐乡市皮肤病医院,浙江桐乡314500

出　　处：《中国卫生检验杂志》2012年第9期2109-2110,2113,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨三种不同的梅毒检测方法在诊断和治疗中的价值。方法:对本院1542例门诊标本同时用梅毒血清酶联免疫吸附试验(ELISA)和梅毒螺旋体明胶凝集试验(TPPA)及甲苯胺红不加热试验(TRUST)检测,阳性标本再用TPPA确证。结果:TPPA确证阳性的159例标本中,ELISA检出158例阳性,检出率99.4%,TRUST检出110例阳性,检出率69.2%,差异具有显著统计学意义;ELISA检测样本OD值的高低与TPPA和TRUST的符合率有显著差异。结论:TRUST仅适合于梅毒治疗的滴度检测及疗效观察,ELISA灵敏度高,适合于筛查,TPPA特异性好,适合于筛查后的确认试验。Objective：To explore the clinical value of the different serological surveys in the diagnosis and treatment of syphilis.Methods： Sera of the 1542 clinical samples in our hospital were tested with ELISA,TRUST and TPPA.The positive samples were confirmed by TPPA.Results： Among the 159 positive samples confirmed by TPPA,158 samples were tested positive by ELISA,the positive rate was 99.4%;while 110 samples were positive with TRUST,the positive rate was 69.2%;the difference of the two methods was statistically significant.The OD values varied distinctively when compared with that of TPPA and TRUST.Conclusion： TRUST is suitable for the determination of the titers and observation of curative effect for syphilis treatment;ELISA is suitable for syphilis screening test due to its sensitivity;and for its specificity,TPPA applies to the process of confirmation most frequently.

关 键 词：梅毒 ELISA TPPA TRUST 

分 类 号：R446.6[医药卫生—诊断学]