Interaction of Human Fibroblasts with Electrospun Composites Gelatin/PLLA,Chitosan/PLLA and PLLA Fibrous Scaffolds  

作　　者：李建波 韩君 任杰 

机构地区：[1]Department of Chemistry Ministry of Education,Tongji University [2]School of Material Science and Engineering Ministry of Education,Tongji University [3]Key laboratory Advanced Civil Engineering Materials,Ministry of Education,Tongji University

出　　处：《Journal of Shanghai Jiaotong university(Science)》2012年第5期559-566,共8页上海交通大学学报（英文版）

基　　金：the Project of the Science and Technology Commission of Shanghai Municipality (No.Ilnm0505100);the China Postdoctoral Science Foundation(No.2012M510116);the Fundamental Research Funds for the Central Universities (No.0500219160)

摘　　要：Highly porous ultrafine electrospun scaffolds,gelatin/poly(L-lactic acid)(PLLA) and chitosan/PLLA were prepared by blending gelatin and PLLA,chitosan and PLLA respectively.The biocompatibilities of these scaffolds were assessed by attachment,proliferation and viability of cells on them.The results indicated that over 30%WI-38 cells could attach to the gelatin/PLLA and chitosan/PLLA scaffolds at 2 h after seeding,while the attachment of the cells was only 15%on PLLA scaffolds.Both gelatin/PLLA and chitosan/PLLA scaffolds also exhibited a very good ability for proliferation of WI-38 cells.Cell growth on the gelatin/PLLA and chitosan /PLLA scaffolds showed dramatic improvement,indicating a much better biocompatibility in the blends contributed by gelatin and chitosan.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay also demonstrated gelatin/PLLA showed better ability to enhance the growth and functions of the cells.These assays suggest that the electrospun gelatin/PLLA and chitosan/PLLA scaffolds are promising biomaterials with great biocompatibility for the development of skin tissue engineering.Highly porous ultrafine electrospun scaffolds, gelatin/poly（L-lactic acid） （PLLA） and chitosan/PLLA were prepared by blending gelatin and PLLA, chitosan and PLLA respectively. The biocompatibilities of these scaffolds were assessed by attachment, proliferation and viability of cells on them. The results indicated that over 30% WI-38 cells could attach to the gelatin/PLLA and chitosan/PLLA scaffolds at 2 h after seeding, while the attachment of the cells was only 15% on PLLA scaffolds. Both gelatin/PLLA and chitosan/PLLA scaffolds also exhibited a very good ability for proliferation of WI-38 cells. Cell growth on the gelatin/PLLA and chi- tosan/PLLA scaffolds showed dramatic improvement, indicating a much better biocompatibility in the blends contributed by gelatin and chitosan. 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay also demonstrated gelatin/PLLA showed better ability to enhance the growth and functions of the cells. These assays suggest that the electrospun gelatin/PLLA and chitosan/PLLA scaffolds are promising biomaterials with great biocompatibility for the development of skin tissue engineering.

关 键 词：poly(L-lactic acid)(PLLA) GELATIN chitosan ELECTROSPINNING FIBROBLAST cell culture 

分 类 号：O636.9[理学—高分子化学] Q253[理学—化学]