压力蒸汽灭菌器灭菌延迟时间的研究  

RESEARCH ON THE STERILIZATION DELAY TIME OF AUTOCLAVE

作　　者：杨祖顺[1] 赵世文[1] 段智泉[1] 罗山[1] 杨萍[1] 田云屏[1] 

机构地区：[1]云南省疾病预防控制中心,昆明650022

出　　处：《中国消毒学杂志》2012年第8期682-684,共3页Chinese Journal of Disinfection

基　　金：国家科技支撑计划课题:实验室污染物泄漏的环境危害评估模型和管理信息系统的研究;编号:2008BAI62B05

摘　　要：目的研究压力蒸汽灭菌器的灭菌延迟时间问题,为科学设定灭菌参数提供依据。方法用动态温度监测仪监测方法,观察不同包装及装载方式下灭菌器内动态温度变化,确定灭菌器达到设定温度所需的延迟时间。结果灭菌器不同位置、不同载量的灭菌延迟时间为0 min;在空载条件下,以标准测试包和生物垃圾袋全开口方式包装,其灭菌延迟时间为0 min;以玻璃瓶、塑料瓶、生物垃圾袋半开口及生物垃圾袋密闭方式包装,其灭菌延迟时间分别为14 min3、5 min、5 min和24 min。在满载复合因素影响下,最长的灭菌延迟时间可达48 min,且若灭菌参数仅设定为121℃3、0 min,不能达到灭菌效果。结论在进行灭菌参数设定时,应考虑压力蒸汽灭菌器的灭菌延迟时间,根据灭菌物品包装及装载方式,调整灭菌参数,否则可能造成灭菌操作失败。Objective To study the sterilization delay time of autoclave in order to provide a basis for setting its sterilization parameters scientifically.Methods The dynamic temperature recorder monitoring method was utilized in the laboratory to monitor the interior dynamic change of temperature under different packing and loading modes,and to determine the sterilization delay time of autoclave.Results The sterilization delay time of autoclave was 0 min under different position or different load.Under no load condition,there was hardly sterilization delay time when temperature sensing probe were packed with standard test kits and full opening plastic bag.However,the sterilization delay time were 14 min,35 min,5 min and 24 min when parked with glass bottle,plastic bottle,half opening plastic bag or sealing plastic bag respectively.The longest sterilization delay time was up to 48 min under complex loading mode.And there was no sterilization efficacy if the sterilization parameters was set as 121℃,30min only.Conclusion The sterilization delay time of autoclave,the packing and loading mode must be considered when operator sets sterilization parameters.The sterilization parameters should be adjusted reasonably,otherwise sterilization operation would fail.

关 键 词：压力蒸汽灭菌器 温度监测 灭菌延迟时间 装载方式 

分 类 号：R187.5[医药卫生—流行病学]