蚕丝蛋白仿生多肽RAGA16的纳米结构与性能研究  

Study on the Nano-Structure and properties of biomimetic peptide RAGA16 bioinspired by natural silk fibroin

作　　者：周庆翰[1] 廖戎[1] 万静[1] 罗建斌[1] 柏松[1] 林娟[2] 

机构地区：[1]西南民族大学化学与环境保护工程学院,成都610041 [2]成都医学院生物医学系,成都610083

出　　处：《四川大学学报（自然科学版）》2012年第5期1101-1106,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：西南民族大学化学一级学科硕士点建设基金;四川省教育厅科研基金项目(10ZC091);西南民族大学中央高校专项资金项目(12NZYQN11;11NZYQN20);成都医学院自然科学基金(CYZ11-010)

摘　　要：利用天然蛋白质氨基酸序列设计生物仿生材料是近年来兴起的一个热门研究领域.本文中将蚕丝蛋白特征氨基酸序列(Gly-Ala-Gly-Ala-Gly-Ser)部分引入离子互补型多肽RADA16-Ⅰ中,设计了新型多肽RAGA16.采用原子力显微镜(AFM)、旋转流变仪(Rheometer)、傅立叶变换红外光谱仪(FTIR)、倒置荧光显微镜等技术对多肽RAGA16的自组装结构、肽链的二级结构、流变学性能以及细胞相容性等进行研究.结果表明,蚕丝蛋白多肽序列引入后,多肽RAGA16具有自组装特性,能够自组装形成致密的纳米级三维网络结构,所形成的水凝胶力学性能明显提高,通过分析得知这可能是由于多肽二级结构中Silk I结构成分比例增加所致.荧光染色显示,绝大多数接种于多肽水凝胶中的小鼠前成骨细胞MC3T3-E1能存活,且在不同的三维平面上生长增殖.综上,利用蚕丝蛋白特征序列对RADA16-Ⅰ进行仿生改性后,对拓展多肽材料在生物医学领域的应用具有重要意义.Designing bio-mimetic materials by mimicing the amino acid sequence of natural proteins has received considerable attention in recent years. A novel self-assembly peptide, RAGA16, was designed by partially inserting the characteristic silk fibroin motif Gly-Ala-Gly-Ala-Gly-Ser into an ionic self-complementary peptide RADA16-I. Modern analysis methods were utilized to study the self-assembly structure, the secondary structure, viscoelastic property, and cell compatibility of the peptide by atomic force microscopy （AFM）, rheometer, fourier transform infrared spectrum （FTIR）, and inverted fluorescence microscope. The novel peptides formed compact network structure in three-dimension, and me- chanical strength of the peptide hydrogel was remarkably improved due to the increase of the silk I structure from inserted fibroin motif segment. Fluorescence staining showed that vast majority of MC3T3-E1 cells in the RAGA16 hydrogel could adhesive, survive, and distribute on different planes. Generally, the characteristic silk fibroin motif was found a critical role in biomimetic modification, which could greatly extend the applications of the peptide in bio-medical field.

关 键 词：蚕丝蛋白 自组装多肽 纳米结构 力学性能 细胞相容性 

分 类 号：TB34[一般工业技术—材料科学与工程]