环介导等温扩增技术在快速检测炭疽芽胞杆菌中的应用  

Loop-mediated isothermal amplification technology in the rapid detection of Bacillus anthracis

作　　者：段圣亮[1] 陆晔[2] 田桢干[2] 王桂江[2] 

机构地区：[1]中国人民解放军防化指挥工程学院,北京102205 [2]上海出入境检验检疫局,上海200335

出　　处：《微生物与感染》2012年第3期152-156,共5页Journal of Microbes and Infections

基　　金：国家质检总局公益项目(200910185)

摘　　要：本文旨在建立适合国境口岸现场应用的生物恐怖防控快速检测方法,从而保障口岸安全。针对生物恐怖炭疽芽胞杆菌,选择目标菌种特异性基因片段,设计引物,运用环介导等温扩增(LAMP)技术建立一套简便、高效的检测方法,并模拟生物恐怖炭疽芽胞杆菌可能存在的基质条件,评价LAMP技术在快速筛查中的适用性。结果显示,LAMP技术排查生物恐怖炭疽芽胞杆菌简便、快速、特异,检测灵敏度为102～103CFU/ml;且能有效检出在偏酸、偏碱及黏稠基质中的炭疽芽胞杆菌。而高盐环境对该反应影响较大,有必要采用能有效去除盐分的核酸抽提方法。The present paper aims to establish a rapid detection method for Bacillus anthracis. The primes were designed according to Bacillus anthracis strain-specific gene fragment, and loop-mediated isothermal amplification （LAMP） was used to establish the detection method. The results showed that LAMP can effectively identify the specific target bacteria with sensitivity of 102^-103^ CFU/ml. It is suggested that LAMP is simple and fast in detection of bioterrorism bacteria such as Bacillus anthracis in acidic, alkaline and viscous media. High-salt environment influences LAMP results, so it is necessary to effectively remove salt out of nucleic acid before application of LAMP.

关 键 词：生物恐怖 环介导等温扩增 炭疽芽胞杆菌 

分 类 号：R440[医药卫生—诊断学]