白细胞介素4对体外培养人黑素细胞增殖及黑素合成的影响  

Effects of Interleukin-4 on Cell Proliferation and Melanin Synthesis in Cultured Human Melanocytes in Vitro

作　　者：陈静宇[1] 林新瑜[2] 王芳[2] 林鸿刚[3] 

机构地区：[1]成都市第六人民医院,成都610051 [2]四川省医学科学院.四川省人民医院,成都610072 [3]四川大学国家生物治疗重点实验室,成都610041

出　　处：《中国中西医结合皮肤性病学杂志》2012年第4期215-218,共4页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

摘　　要：目的建立正常人表皮黑素细胞体外培养体系,观察不同浓度白细胞介素4(interleukin-4,IL-4)对体外培养正常人黑素细胞的细胞增殖及黑素合成的影响。方法采用四甲基偶氮唑盐比色(methylthiazolyl tetrazolium,MTT)法测定不同浓度IL-4对黑素细胞增殖的影响,氢氧压钠(NaOH)裂解法测定黑素生成量,流式细胞仪检测黑素细胞的凋亡率。结果 IL-4对黑素细胞活力有抑制作用,能使细胞增殖能力降低,黑素合成减少,增加黑素细胞的凋亡率。结论 IL-4对体外培养的黑素细胞增殖及黑素生成都有抑制作用,这为临床应用IL-4治疗色素性疾病提供了实验依据。Objective To establish the cultured system of human normal melanocytes in vitro,and to study effects of interleukin-4（IL-4） on cultured human melanocytes.Methods Methyl thiazolyl tetrazolium（MTT） method,NaOH cracking process assay was employed to measure the rate of proliferation,melanin synthesis of melanocytes respectively;apoptosis rates of melanocytes were detected by flow cytometry.Results IL-4 showed a significant decreasing in the rate of melanocyte proliferation in concentration and time-dependent manner.It could inhibite melanin synthesis and increase melanocyte apoptosis.Conclusion It suggests that IL-4 at the concentration of 50 to 200 μg/L is able to suppress melanocytic proliferation and melanin synthesis.This provides experiment reference for IL-4 on treating pigmentary diseases.

关 键 词：白细胞介素4 黑素细胞 

分 类 号：R758.4[医药卫生—皮肤病学与性病学]