自制小动物定量创伤仪对大鼠心肌组织损伤的观察  

Observation of myocardial tissue injuries caused by a self-made,small animal quantitative trauma device

作　　者：刘宣[1] 吕广艳[2] 吕国枫[3] 于德钦[1] 陈小龙[1] 陈冲[1] 李树壮[1,3] 

机构地区：[1]大连医科大学生理学教研室,辽宁大连116044 [2]大连医科大学中心实验室,辽宁大连116044 [3]大连医科大学运动医学教研室,辽宁大连116044

出　　处：《心脏杂志》2012年第5期583-587,590,共6页Chinese Heart Journal

基　　金：国家自然科学基金项目资助(30971065);辽宁省教育厅基金(2009A194);大连市科技计划项目(2012E12SF074)

摘　　要：目的:观察使用自制小动物定量创伤仪,造成的大鼠全身机械性创伤。方法:首先设计并组装制造实验用小动物定量全身性机械性创伤仪。将40只大鼠分为对照组和创伤组,每组20只(n=20),以40 r/min的转速旋转200圈,分别给予创伤组动物定量创伤,观察心肌组织中心肌细胞的凋亡指数、Caspase-3、iNOS、NO、NADPH氧化酶以及超氧阴离子(.O2-)的表达量,对照组未做任何处理。结果:创伤组心肌细胞的凋亡指数、Caspase-3以及iNOS的表达、NO的产生、NADPH氧化酶的表达及.O2-的生成,均明显高于对照组(P<0.01)。结论:使用自制小动物创伤仪,定量给予大鼠全身性机械性创伤,可造成大鼠心肌细胞发生明显的氧化应激反应和凋亡。AIM： To observe myocardial tissue injury caused by a self-made, small animal quantitative trauma device. METHODS： Forty rats were randomly divided equally into two groups： control group （n = 20） and trauma group （n = 20）. Rats of the trauma group were anesthetized with pentobarbital sodium （40 mg/kg）, individually placed on a self-made small animal quantitative trauma device and were traumatized as the wheel rotated （200 revolutions （r） at a rate of 40 r/min）. Measures were taken to observe the pathological changes of myocardial tissue. RESULTS： Compared with those in control group, the apoptotic index of the myocardial cells was elevated （ P 〈 0. 01 ）. Activity of caspase-3, expression of both iNOS and NADPH oxidase, and production of both NO and .O2^- （ superoxide anion） were also increased in the trauma group （ all P 〈 0. 01 ）. CONCLUSION ： Mechanical trauma may induce significant oxidative stress and apoptosis in rat myocardial tissues by using a self-made, small animal quantitative trauma device.

关 键 词：机械性损伤 创伤仪器 心肌 大鼠 

分 类 号：R542.2[医药卫生—心血管疾病]