Effects of total dissolved gas supersaturated water on lethality and catalase activity of Chinese sucker(Myxocyprinus asiaticus Bleeker)  

作　　者：Shi-chao CHEN Xiao-qing LIU Wen JIANG Ke-feng LI Jun DU Dan-zhou SHEN Quan GONG 

机构地区：[1]State Key Laboratory of Hydraulics and Mountain River Engineering,Sichuan University,Chengdu 610065,China [2]School of Energy and Environment,Xihua University,Chengdu 610039,China [3]Fisheries Institute,Sichuan Academy of Agricultural Sciences,Chengdu 611731,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2012年第10期791-796,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation ofChina (No. 50979063);the Scientific Research Foundation for Young Teachers of Xihua University (No. Z1120412),China

摘　　要：Total dissolved gas(TDG) supersaturation caused by dam sluicing can result in gas bubble trauma(GBT) in fish and threaten their survival.In the present study,Chinese suckers(Myxocyprinus asiaticus Bleeker) were exposed to TDG supersaturated water at levels ranging from 120% to 145% for 48 h.The median lethal concentration(LC 50) and the median lethal time(LT 50) were determined to evaluate acute lethal effects on Chinese suckers.The results showed that the LC 50 values of 4,6,8,and 10 h were 142%,137%,135%,and 130%,respectively.The LT 50 values were 3.2,4.7,7.8,9.2,and 43.4 h,respectively,when TDG supersaturated levels were 145%,140%,135%,130%,and 125%.Furthermore,the biological responses in Chinese suckers were studied by assaying the catalase(CAT) activities in gills and muscles at the supersaturation level of 140% within LT 50.The CAT activities in the gills and muscle tissues exhibited a regularity of a decrease after an increase.CAT activities in the muscles were increased significantly at 3/5LT 50(P<0.05) and then came back to the normal level.However,there were no significant differences between the treatment group(TDG level of 140%) and the control group(TDG level of 100%) on CAT activities in the gills before 3/5LT 50(P>0.05),but the activities were significantly lower than the normal level at 4/5LT 50 and LT 50(P<0.05).Total dissolved gas（TDG） supersaturation caused by dam sluicing can result in gas bubble trauma（GBT） in fish and threaten their survival.In the present study,Chinese suckers（Myxocyprinus asiaticus Bleeker） were exposed to TDG supersaturated water at levels ranging from 120% to 145% for 48 h.The median lethal concentration（LC 50） and the median lethal time（LT 50） were determined to evaluate acute lethal effects on Chinese suckers.The results showed that the LC 50 values of 4,6,8,and 10 h were 142%,137%,135%,and 130%,respectively.The LT 50 values were 3.2,4.7,7.8,9.2,and 43.4 h,respectively,when TDG supersaturated levels were 145%,140%,135%,130%,and 125%.Furthermore,the biological responses in Chinese suckers were studied by assaying the catalase（CAT） activities in gills and muscles at the supersaturation level of 140% within LT 50.The CAT activities in the gills and muscle tissues exhibited a regularity of a decrease after an increase.CAT activities in the muscles were increased significantly at 3/5LT 50（P0.05） and then came back to the normal level.However,there were no significant differences between the treatment group（TDG level of 140%） and the control group（TDG level of 100%） on CAT activities in the gills before 3/5LT 50（P0.05）,but the activities were significantly lower than the normal level at 4/5LT 50 and LT 50（P0.05）.

关 键 词：Total dissolved gas supersaturation Median lethal time(LT 50) Median lethal concentration(LC 50) Chinese sucker CATALASE 

分 类 号：Q17[生物学—水生生物学]