陕西地区老年动脉粥样硬化群体Rb基因XbaI酶切位点多态性研究  

Rb gene XbaI polymorphism in relation to atherosclerosis in Shaanxi elder population

作　　者：刘军[1] 舒青[1] 郑强荪[1] 杜日映[1] 张宁仔[1] 

机构地区：[1]第四军医大学,唐都医院心脏内科陕西西安710038

出　　处：《心脏杂志》2000年第3期196-197,200,共3页Chinese Heart Journal

基　　金：国家自然科学基金!No.3970 0165

摘　　要：目的 :探讨陕西地区老年动脉粥样硬化 (AS)群体 Rb基因内含子 17多态性分布 ,及其该位点与 AS遗传易感性的关系。方法 :应用 PCR对 10 0例老年 AS患者及 10 0例对照组老年人 Rb基因内含子 17Xba I酶切位点的限制性片段长度多态性 (RFL P)进行对比分析。结果 :在 AS组及对照组中该位点共检出 2种等位基因片段 :945 bp(S1 )及 6 30 bp加 315 bp(S2 ) . AS组基因频率 S1:0 .46 ,S2 :0 .5 4,对照组基因频率 S1 :0 .42 ,S2 :0 .5 8;二者的等位基因频率无显著差异。结论 :Rb基因内含子 17Xba I酶切位点在 AS群体中稳定性较高 ,不易发生突变 ,对 AS的形成。AIM: To investigate the relationship between the polymorphism of Rb (intron17) in elder population of Shaanxi and atherosclerosis(AS) genetic susceptibility. METHODS: The polymorphism of Rb (intron17) were examined in 100 elder AS individuals and 100 elder control samples in Shaanxi by using PCR Rb XbaI RFLP. RESULTS: There are 2 alletic fragments (945 bp, 630 bp+315 bp) were found in AS group and control group. S 1S 2 genotype was the most frequent one in the two populations. Allele frequency of AS group is S 1 0.46, S 2 0.54 and that of control group is S 1 0.42,S 2 0.58. Allele frequency was no differences in the two groups ( P >0 05). CONCLUSION: XbaI site of Rb (intron17) could have been certainly stable in AS population, we infered it was not liable to cause mutation, and the locus might not make a notable impact to the occurrence and development of AS.

关 键 词：动脉粥样硬化 RB基因 限制性片段长度多态性 

分 类 号：R543.502[医药卫生—心血管疾病]