电刺激诱导栉孔扇贝雌核发育的相关参数的筛选  

Determination of the Electrical Pulsing Parameters Inducing the Gynogenesis of Chlamys farreri

作　　者：商文聪[1] 赵泊淞[1] 陈亮[1] 孙妍[1] 张玲玲[1] 胡晓丽[1] 包振民[1] 

机构地区：[1]中国海洋大学贝类分子遗传学与育种技术研究室,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2012年第10期65-70,共6页Periodical of Ocean University of China

基　　金：国家自然科学青年基金项目(30901096);国家高技术研究发展计划(2012AA10A402);国家科技支撑计划项目(2011BAD13B06;2011BAD13B05);现代农业产业技术体系建设专项资金;山东省农业良种工程资助

摘　　要：电刺激可诱导卵子雌核发育,但在海洋生物中尚无报道。为获得电刺激诱导栉孔扇贝雌核发育的最佳条件,采用正交设计的方法,分别研究了电场强度、电容、脉冲次数、卵子密度及缓冲液种类对诱导效果的影响。结果表明:(1)不同缓冲液的诱导效果差异不显著(P>0.05),但甘露醇缓冲液的诱导效果优于Zimmerman氏缓冲液和山梨醇缓冲液;最佳卵子密度为11.25×104个/mL,场强的最适范围为1.00～1.75kV/cm。最优电刺激诱导条件组合是:0.30mol/L甘露醇缓冲液,卵子密度11.25×104个/mL,脉冲强度1.20kV/cm,电容3μF,2次电脉冲。(2)应用优化后的电刺激参数诱导雌核发育,获得了最佳的诱导效果,得到的卵裂率为28.16%,胚胎存活率为98.37%。本研究首次成功应用电刺激法诱导了栉孔扇贝的雌核发育,并得到了单倍体胚胎,初步建立了栉孔扇贝中该诱导法的参数体系。Electrical pulse induces the gynogenesis of diverse organisms; however such an induction was not tried in marine species. In this study, the orthogonal design was adopted to determine the electrical pulsing parameters （electric field strength, electric capacity, density of eggs, electro-activation solution and pulse number） for inducing the gynogenesis of Chlamys farreri. We found that 0. 30 mol/L mannitol supplemented electro-activation solution, 11.25×10^4 eggs/mL, electric 1.20kv/cm field strength and 3 F electric capacity and two times of pulsing were optimal, under which a cleavage rate of 28. 16% and a sur-vival rate of 98. 370% gynogenetic embryos were reached. This was the first successful trial of inducing gy- nogenesis with electrical pulse in C. farreri.

关 键 词：栉孔扇贝 雌核发育 电刺激 卵裂率 胚胎存活率 

分 类 号：Q953.1[生物学—动物学]