中药抗癌方诱导人结肠癌细胞株细胞凋亡的实验研究  

Experiment Study of Influence on Apoptosis of Human Colon Cancer Cell Line with Chinese Traditional Medicine Kang Ai Fang

作　　者：陈泽雄[1] 张诗军[1] 陈雯[2] 孔庆瑜[1] 

机构地区：[1]中山医科大学附属第一医院中医科,广州510080 [2]中山医科大学卫生学院,广州510080

出　　处：《中国中西医结合脾胃杂志》2000年第3期135-136,共2页

基　　金：广东省中医药管理局科研基金资助项目!(No.97230)

摘　　要：目的 :研究中药抗癌方 (KAF)对人结肠癌细胞凋亡的影响。方法 :选择人结肠癌细胞株 HB86 93作为靶细胞 ,用 MTT掺入试验测定细胞毒作用 ,用流式细胞仪检测计算 HC86 93细胞株的亚 G1峰细胞 (凋亡 )的百分率 ,并与空白对照组比较。结果 :KAF对 HC86 93的 L D5 0 为 2 5 .4% ;在 5 %及 10 %浓度作用于 HC86 93后的亚 G1峰细胞百分数分别为 (2 2 .0± 2 .6 ) %和 (2 9.5± 3.1) % ,与空白对照组 (6 .3± 1.0 ) %相比 ,皆有极显著性差异 (P<0 .0 1)。结论 :KAF能诱导人结肠癌细胞凋亡。Objective:To understand effect of inducing apoptosis of human colon cancer cells with Kang Ai Kang(KAF).Methods:Human colon cancer cell line HC8693 was selected as target cells.The cytotoxic effect of KAF on tumor cells was quantified using MTT colorimetric assay.The percentage of sub Gl apex cells of HC8693 cell line was checked and counted by FACS.Results:The LD 50 of KAF on HC8693 cell line was 25.4%.The percentage of sub Gl apex cells were (22.0±2.6)% and(29.5±3.1)% while the medicine concentration were five percent and ten percent.They had markedly significance as compared with the blank contral group (6.3±1.0)%(P< 0.05 ).Conclusion:The KAF could influence apoptosis of human colon cancer cells.

关 键 词：抗癌方 人结肠癌细胞株 细胞凋亡 中医药疗法 

分 类 号：R273.535[医药卫生—中西医结合]