一起由肠侵袭性大肠埃希菌O136∶K78引起的聚集性腹泻暴发  

An outbreak of diarrhea caused by enteroinvasive Escherichia coli O136∶K78

作　　者：夏文[1] 薛琳[1] 周显凤[1] 樊国印[1] 贺凤兰[1] 甘启芦[1] 

机构地区：[1]南昌市疾病预防控制中心,江西南昌330038

出　　处：《疾病监测》2012年第9期726-728,共3页Disease Surveillance

摘　　要：目的明确聚集性食源性腹泻暴发的病原。方法根据现场流行病学调查,对患者肛拭子标本18份,粪便标本2份,厨师粪便标本1份,进行分离培养,对分离到的可疑病原菌进行生化鉴定,采用Real-time PCR技术检测可疑病原菌的ipaH毒力基因,同时进行脉冲场凝胶电泳(PFGE)分型分析。结果分离到11株血清型为O136∶K78的肠侵袭性大肠埃希菌,除1份肛拭子外,其余7份患者肛拭子、3份粪便标本Real-time PCR均阳性;除1株菌外,其余10株菌株的PFGE带型一致。结论具有侵袭性相关基因ipaH的O136∶K78肠侵袭大肠埃希菌是本次聚集性腹泻暴发的主要原因。Objective To determine the pathogen of a foodborne outbreak of diarrhea.Methods On the basis of epidemiological investigation,eighteen rectal swabs and 2 stool samples from the patients and 1 stool sample from a cook were collected.After pathogen isolation and culture,the biochemical identification of the isolated strains was performed,the invasive plasmid antigen H gene（ipaH） of the positive strains were detected by real-time PCR and the pulsed-field gel electrophoresis homology analysis was conducted.Results Eleven strains of enteroinvasive Escherichia coli O136∶K78 were isolated.All the samples,except 1 rectal swab,were positive in real time PCR,and the PFGE patterns of all the strains,except one,were consistent.Conclusion The enteroinvasive Escherichia coli O136∶K78 with ipaH gene was the pathogen to cause the outbreak of diarrhea.

关 键 词：侵袭性大肠埃希菌 O136∶K78 ipaH 腹泻 

分 类 号：R378.21[医药卫生—病原生物学]