纳米氧化锌对大鼠血脂和血清炎症因子水平的影响  

Effects of zinc oxide nanoparticles on serum lipid and inflammatory mediator levels in rats

作　　者：梁宁[1] 李冰[1] 燕贞[1] 王威[1] 吴逸明[1] 吴卫东[1] 

机构地区：[1]郑州大学公共卫生学院劳动卫生学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2012年第5期626-628,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目38072148

摘　　要：目的:探讨纳米氧化锌对动脉粥样硬化发生发展的影响机制。方法:40只雄性Wistar大鼠随机分为对照组和纳米氧化锌低、中、高剂量组,每组10只。纳米氧化锌低、中、高剂量组大鼠分别用1.25、2.50、5.00mg/kg的纳米氧化锌悬浮液气管灌注染毒,对照组大鼠同法灌注PBS溶液(1mL/kg),每周1次,共12周;染毒结束后测定各组大鼠体质量、血清总胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、肿瘤坏死因子α(TNF-α)和血红素加氧酶-1(HO-1)水平。结果:随染毒时间延长,染毒大鼠逐渐出现毛发蓬松,活动减少,精神状态差,生长缓慢等体征。各组大鼠血清HDL、LDL、TNF-α、HO-1、TC水平比较差异有统计学意义(F=32.410、34.600、23.957、38.519、32.652,P<0.001)。纳米氧化锌中、高剂量组大鼠血清TC、LDL、HO-1、TNF-α水平均高于对照组(P<0.05);纳米氧化锌低、中、高剂量组大鼠血清HDL水平均低于对照组(P<0.05)。结论:纳米氧化锌可引起大鼠血清脂代谢紊乱和炎症因子释放,可能导致动脉粥样硬化的发生发展。Aim： To detect the levels of serum lipid metabolic and inflammation mediator in rats intratracheally instilled with zinc oxide nanoparticles.Methods：A total of 40 male Wistar rats were randomly divided into four groups：the control group,and zinc oxide nanoparticles low,medium,high dose groups. The rats of zinc oxide nanoparticles low,medium,high dose groups were instilled with 1.25,2.50,5.00 mg/kg zinc oxide nanoparticles,respectively.And the control group were given PBS. Rats were intratracheally instilled with zinc oxide nanoparticles once a week. After 12 weeks, body weight and the levels of serum TC, LDL, HDL, TNF-α and HO-1 were determined.Results：With exposure continued, rats in the experimental groups presented rough fur, inactive, poorer mental state, and slow growth.The levels of serum HDL,LDL,TNF-α,HO-1 and TC among the 4 groups were different（F=32.410,34.600,23.957,38.519,32.652,P0.001）. Levels of serum TC,TNF-α,LDL and HO-1 in zinc oxide nanoparticles medium,high dose groups were higher than those in the control group （P0.05 ）.Levels of serum HDL in zinc oxide nanoparticles low,medium,high dose groups were lower than those in the control group（P0.05）.Conclusion：Intratracheal instillation of zinc oxide nanoparticles could induce lipid metabolic disorders and inflammation mediator release,and these changes in serum have certain effects on atherosclerosis.

关 键 词：纳米氧化锌 动脉粥样硬化 脂蛋白 炎症因子 大鼠 

分 类 号：R136.3[医药卫生—劳动卫生]