咯利普兰对肺纤维化大鼠内皮素-1水平的影响  

Effect of rolipram on lever of endothelin-1 in rats with pulmonary fibrosis

作　　者：潘金兵[1] 侯宇虹[2] 钱皓喻[1] 

机构地区：[1]河南省人民医院呼吸科,郑州450003 [2]郑州大学第一附属医院急诊科,郑州450052

出　　处：《郑州大学学报（医学版）》2012年第5期710-713,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:观察喀利普兰对肺纤维化大鼠内皮素-1(ET-1)含量的影响。方法:48只Wistar大鼠随机分为对照组、模型组和喀利普兰组,对照组腹腔注射体积分数为5%乙醇的生理盐水,后2组制备模型前一天腹腔分别注射体积分数为5%乙醇的生理盐水(0.5mL)和喀利普兰溶液(0.25mg·kg-1·d-1);分别于第7、28天收集标本,测定肺匀浆ET-1和羟脯氨酸(HYP)含量。结果:造模后第7、28天,3组大鼠肺匀浆中ET-1含量差异均有统计学意义(F=9.749,8.771,P<0.05)。模型组肺匀浆ET-1含量[(98.82±15.25)mg/g,72.88±22.59)mg/g]均高于对照组[(65.72±17.67)mg/g,(36.00±15.49)mg/g,P<0.05)];而喀利普兰组[(78.03±12.02)mg/g,(49.05±14.38)mg/g]低于模型组(P<0.05)。第28天,喀利普兰组肺HYP含量[(1.316±0.225)mg/g]低于模型组[(2.399±0.152)mg/g)](P<0.05)。结论:喀利普兰对平阳霉素诱发的肺纤维化有阻断作用,可能是通过拮抗ET-1而实现。Aim ：To investigate the level of endothelin-1 （ ET-1 ） in with pulmonary fibrosis rats intervened by rolipram. Methods：A total of 48 Wistar rats were randomly divided into three groups：the control group, the model group and rolip- ram group,and the latter two groups were injected into intraperitoneally with 5% alcohol normal saline and rolipram （0.25 mg ± kg-1 · d-1 ） each before injecting pingyangmycin via trachea, while the control group was injected with 5% alcohol normal saline and NS. At the 7th and 28th day,lung samples were collected to determine the level of hydroxyproline（HYP） and the level of ET-1. Results：At 7th and 28 th day,the level of ET-1 in the control group （65.72 ± 17.67） mg/g, （36.00 ± 15.49） mg/g] were lower than those in the model group[ （98.82 ± 15.25） mg/g,（72.88 ±22.59） mg/g,P 〈 0.05 ） ], which were higher than the rolipram group （78.03 ± 12.02 ） rag/g, （ 49.05 ± 14.38 ） mg/g, P 〈 0.05 ） ]. At the 28th day,the HYP content in the model group was higher than the rolipram group. Conclusion： Rolipram can inhibit pul- monary fibrosis induced by pingyangmycin through inhibiting the level of ET-1.

关 键 词：喀利普兰 肺纤维化 内皮素-1 大鼠 

分 类 号：R563.9[医药卫生—呼吸系统]