电针对抑郁模型大鼠五羟色胺及脑源性神经营养因子的影响  

Effects of electroacupuncture on the expression of 5-hydroxytryptamine and brain-derived neurotrophic factor in depression rats

作　　者：史榕荇[1] 吴茜[2] 于战歌[1] 李辉[1] 田旭升[2] 唐学章[1] 

机构地区：[1]中日友好医院针灸科,北京100029 [2]黑龙江中医药大学,哈尔滨150040

出　　处：《中日友好医院学报》2012年第5期287-289,共3页Journal of China-Japan Friendship Hospital

摘　　要：目的:观察电针对抑郁模型大鼠5-羟色胺(5-HT)、脑源性神经营养因子(BDNF)表达水平的影响,探讨电针改善大脑功能可能存在的机制。方法:采用慢性应激抑郁模型,将大鼠分为空白对照组、抑郁模型组、阳性西药组、电针治疗组(以电针刺激百会、印堂穴);Elisa法检测各组大鼠海马内5-HT含量、Western-blot法检测海马内BDNF蛋白表达水平。结果:给予慢性应激刺激后,模型组大鼠海马5-HT含量较正常组显著减少(P<0.01),电针组大鼠海马5-HT含量较模型组显著增加(P<0.01)。Western-blot印迹结果显示与正常组相比,模型组BDNF蛋白表达量显著降低(P<0.01);电针组BDNF蛋白表达量较模型组显著增加(P<0.01)。结论:提高海马内5-HT含量及BDNF表达,增强神经可塑性是电针抗抑郁可能的机制。Objective ：To investigate the relationship of electroacupuncture（EA）enhance the capability of neural plasticity in depression rats by increasing the expression of brain-derived neurotrophic factor （BDNF） and 5- hydroxytryptamine （5-HT）.Methods：Adopt chronic stress depression model rats and stimulate them on Baihui, Yintang with EA.Rats were divided into four groups：control group,model group,EA group and fluoxetine treat- ment group.Compared with fluoxetine treatment group,Elisa and Western-blot methods were used to investigate the influence of EA on 5-HT levels and BDNF genetic expression.Results：EA can increase the 5-HT level in hippocampal and enhance its BDNF protein expression at the same time.Conclusion：The enhancement of the protein expression of BDNF and 5-HT maybe the anti-depression mechanism of EA.

关 键 词：抑郁症:电针 脑源性神经营养因子 

分 类 号：R245[医药卫生—针灸推拿学]