HPLC法测定多烯紫杉醇脂质体药物包封率  

Analysis of Entrapment Efficiency of Docetaxel in Docetaxel Liposomes by HPLC

作　　者：吴卫[1,2] 程怡[1] 吴琼[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]桂林医学院,广西桂林541004

出　　处：《辽宁中医杂志》2012年第10期2026-2027,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30772790)

摘　　要：目的:建立多烯紫杉醇脂质体药物包封率测定方法。方法:采用HPLC法测定脂质体中多烯紫杉醇的含量及包封率,色谱柱:Diamonsil C18(250mm×4.6 mm,5μm);流动相:乙腈-水(55∶45);柱温:30℃;流速:1.0mL.min-1;紫外检测波长:230nm。结果:多烯紫杉醇在20.4～204μg·mL-1范围内线性关系良好(r=0.9997),平均回收率为97.59%,RSD为1.53%。结论:该方法简单、快速、准确,可用于多烯紫杉醇脂质体包封率的测定。Objective ： To establish a determination method for entrapment efficiency of docetaxel in docetaxel liposomes. Meth- ods：The content and entrapment efficiency was determined by HPLC. Chromatographic column：Diamonsil C18 （250mm × 4.6mm, 5 μm） ; Mobile phase ： acetonitile-water（ 55 ： 45 ） ; Column temperature ： 30℃ ; Velocity ： 1.0mL · min - 1 ; Detected wavelength ： 230 nm. Results：The linear range for docetaxel was 20. 4 -204μg · mL^-1 （r =0. 9997）. The average recovery was 97.59% and RSD was 1.53%. Conclusion：This determination method is simple, raipd and accurate, can be used for the analysis of entrapment effi- ciency of docetaxel in docetaxel liDosomes.

关 键 词：多烯紫杉醇 脂质体 包封率 

分 类 号：R284.1[医药卫生—中药学]