PNIPAAm-g-EVA/明胶支架双层人工皮肤的细胞相容性研究  

Cell biocompatibility of PNIPAAm-g-EVA/gelatin bilayer artificial skin

作　　者：王妍媖[1] 张利[2] 饶含兵[1] 王晓燕[2] 龚梅[2] 周名兵[2] 李玉宝[2] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014 [2]四川大学分析测试中心,四川成都610064

出　　处：《功能材料》2012年第19期2629-2632,2637,共5页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(50903052);国家重点基础研究发展计划(973计划)资助项目(2012CB933902)

摘　　要：构建了PNIPAAm-g-EVA/明胶支架复合人工皮肤样品,并初步考察了该人工皮肤的细胞相容性。由于明胶支架的生物相容性已经得到充分验证,主要选择成纤维细胞为实验细胞,PNIPAAm-g-EVA膜为研究对象。采用含有胶原酶的消化液消化组织块,在较短时间内可获得足够数量的成纤维细胞。成纤维细胞与PNIPAAm-g-EVA膜共培养结果表明,成纤维细胞在膜表面粘附力强,生长、增殖情况良好。说明与PNIPAAm-g-EVA膜共培养并未影响细胞的正常生理功能,PNIPAAm-g-EVA具有良好的细胞相容性。In this study a gelatin/PNIPAAm-g-EVA bilayer artificial skin had been constructed and its cell com- patibility had also been assessed. As the good biocompatibility of gel had already been proved. So we focus on assessing the cell compatibility of PNIPAAm-g-EVA membrane. Rat skin fibroblasts were cultured in vitro with PNIPAAm-g-EVA membrane for different periods, and the results indicated that the proliferation of fibro- blasts on PNIPAAm-g-EVA membrane increased with time significantly. Imnunohistochemical staining of fi- broblasts were positive. Therefore, it can be concluded that the PNIPAAm-g-EVA membrane has good cyto- compatibility on the basis of positive effect on the attachment, proliferation and growth of fibroblasts.

关 键 词：PNIPAAm-g-EVA 明胶 人工皮肤 成纤维细胞 生物相容性 

分 类 号：O632.6[理学—高分子化学]