钛材料表面固定多聚赖氨酸-肝素纳米颗粒以改善血液相容性的研究  

Immobilization of poly-l-lysine/heparin nanoparticle on titanium surface to improve the blood compatibility

作　　者：胡珂[1] 刘涛[1] 刘诗卉[1] 陈俊英[1] 黄楠[1] 

机构地区：[1]西南交通大学材料科学与工程学院,材料先进技术教育部重点实验室,四川成都610031

出　　处：《功能材料》2012年第19期2685-2688,2693,共5页Journal of Functional Materials

基　　金：国家重点基础研究发展计划(973计划)资助项目(2011CB606204);国家自然科学基金资助项目(31170916)

摘　　要：通过将多聚赖氨酸(PLL)-肝素纳米颗粒固定在多巴胺涂覆的钛表面,以改善其血液相容性。利用zeta电位仪及甲苯胺蓝法检测纳米颗粒的粒径及成分,通过傅立叶变换红外光谱(FT-IR)、X射线光电子能谱(XPS)及水接触角等对颗粒固定前后表面理化性质的变化进行表征。通过体外血小板粘附实验、肝素释放及活化部分凝血活酶时间(APTT)检测对改性样品的血液相容性进行评价。结果表明,PLL-肝素纳米颗粒成功固定在多巴胺沉积的钛表面,纳米颗粒的固定有效降低钛材料表面血小板的粘附行为,大大提高了血液相容性。For improving the blood compatibility of titanium surface, this work mainly develop a new biological surface modification approach. Firstly, the nanoparticles are obtained by dropping heparin into the PLL solu- tions in the supersonic vibrating condition. Then, the PLL-heparin nanoparticles were immobilized onto the do- pamine-coated titanium surface. The particle size and components of the nanoparticles are detected by zeta po- tential analyzer and the toluidine blue method. The physical and chemical properties of the modified titanium surface are characterized with FT-IR, XPS and water contact angle test. Furthermore, the blood compatibility of the modified sample is evaluated by in vitro platelet adhesion experiment, heparin release test and APTT measurement. The results demonstrate that the PLL-heparin nanopartieles can be successfully immobilized to the dopamine-coated titanium surface. The platelet adhesion behavior can be efficiently controlled due to the im- mobilized particles. As a result, the blood compatibility of Ti surface was significantly improved.

关 键 词：钛 多巴胺 多聚赖氨酸 肝素 纳米颗粒 血液相容性 

分 类 号：R318.08[医药卫生—生物医学工程]