泡状带绦虫线粒体部分基因克隆及序列比对分析  

Cloning and sequence analysis of partial mitochondrial genes of Taenia hydatigena

作　　者：项海涛[1] 尚清炎[1] 郝宝成[2] 邢小勇[1] 梁剑平[2] 胡永浩[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所

出　　处：《中兽医医药杂志》2012年第5期9-11,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：自选项目

摘　　要：采集甘肃兰州市区宠物犬体内两株泡状带绦虫,运用分子生物学方法对其线粒体部分基因进行克隆,并使用基因分析软件Larsergene V7.1进行序列分析,结果发现这两株虫体同源性达到99.6%,与国内的泡状带绦虫广东虫株和甘肃虫株同源性分别达到98.7%和98.4%,鉴定为泡状带绦虫;与其他带科绦虫同源性均高于85%,低于88%。其中,与国内牛带绦虫同源性最高,为88%;与国内巨颈带绦虫同源性最低,只有85.1%。推断不同带科绦虫的cox1基因种间差异明显,在带科绦虫的分类上意义较大。Partial mitochondrial genome of two strains of Taenia hydatigena in dogs selected in anzhou were amplified by PCR. Their sequence was analyzed with the software Larsergene V7.1 and compared with tile corresponding sequence of Guangdong strain and Gansu strain published in GenBank.It revealed that the two stratus shared 99.6% of sequence similarity, and had a homology of 98.7% and 98.4% with Guangdong strain and Gansu strain, respecfively. Taenia hydatigena in dogs in Lanzhou had a homology of 85%-88% with the other Taeniidae cestodes. It is conch,deal that coxl gene with species-specific may have great significance in the classification of different Taeniidae cestodes.

关 键 词：泡状带绦虫 线粒体 序列比对 

分 类 号：S855.94[农业科学—临床兽医学]