干旱胁迫对高粱苗期抗旱生理特性的影响  

Effect of drought stress on physiological characteristics of sorghum at seedling stage

作　　者：解芳[1,2] 翟国伟[2] 邹桂花[2] 陶跃之[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021

出　　处：《浙江农业学报》2012年第5期753-757,共5页Acta Agriculturae Zhejiangensis

基　　金：国家自然基金(31000739)

摘　　要：从654/LTR108重组自交系群体中筛选出一个持绿性株系RIL-1和一个早衰株系RIL-2。以这两个株系及其亲本为试验材料,研究了干旱胁迫对其苗期叶片的保护酶活性和渗透调节物质的影响。结果表明,随着干旱胁迫时间的延长,RIL-1,RIL-2,654和LTR108游离脯氨酸含量均上升,且上升幅度RIL-2>LTR108>654>RIL-1;654和RIL-1叶绿素含量先升高后降低,而RIL-2和LTR108先降低后上升再降低;LTR108,654,RIL-2和RIL-1的总抗氧化能力(T-AOC)先升高后降低,但不同基因型抗氧化能力达到峰值的时间有所不同。在胁迫处理的第3天,LTR108和RIL-1的抗氧化能力最高,而654和RIL-2在第4天达到最高;LTR108,654,RIL-2和RIL-1的总超氧化物歧化酶(T-SOD)先升高后降低,且RIL-1的T-SOD变幅最明显;654,RIL-2和RIL-1的过氧化物酶(POD)活性先升高后降低,而LTR108的POD活性先降低后升高再降低,654的POD活性变幅最大。A stay-green line （RIL-1） and an early-senescence line （RIL-2） were selected from a recombinant inbred line population of 654/LTR108. Five physiological indices were investigated from the leaf samples collected from the seedlings of RIL-1, RIL-2,654 and LTR108 under drought stress, respectively. The level of proline content increased significantly with the prolonging of drought stress, RIL-2 had the highest level of proline content, followed by LTRI108, 654 and RIL-1. The chlorophyll content of 654 and RIL-1 firstly increased and then decreased while RIL-2 and LTR108 exhibited a down-up-down changing pattern. The level of T-AOC activity followed an up-down pattern for all the lines investigated. RIL-2 and LTR108 had the highest level of T-AOC activities at the 3rd day, while RIL-1 and 654 reached the highest level at the 4th day. T-SOD activity had a similar pattern as T-AOC activi- ty. The significant change was observed for RIL-1. Different patterns were observed for the level of the POD,activity among 654, RIL-1, RIL-2 and LTR108, up-down pattern for 654, RIL-1 and RIL-2 but down-up-down pattern for LTR108, respectively. The significant change was occurred for the line of 654.

关 键 词：高粱 苗期 抗旱 生理特性 

分 类 号：S514[农业科学—作物学]