高效液相色谱法测定防感喷雾剂中次野鸢尾黄素的含量  

Determination of Irisflorentin in Fanggan Spray by HPLC

作　　者：李甲英[1] 翟玉[1] 王泽锋[2] 李姝梅[1] 韦静[1] 李安华[1] 

机构地区：[1]云南中医学院,云南昆明650500 [2]云南省红河学院,云南红河661100

出　　处：《儿科药学杂志》2012年第10期43-45,共3页Journal of Pediatric Pharmacy

基　　金：云南省科技项目;项目编号2008IF014

摘　　要：目的:建立高效液相色谱(HPLC)测定防感喷雾剂中次野鸢尾黄素含量的方法。方法:采用HPLC进行测定,色谱柱为Agilent C18柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.2%磷酸(60∶40);检测波长266 nm;流速1.0 mL/min;柱温30℃。结果:次野鸢尾黄素在0.026～0.260 mg/mL范围内线性关系良好(r=1.000),平均加样回收率为97.12%,RSD为1.85%。结论:本方法简便、准确、可靠,可用于防感喷雾剂中次野鸢尾黄素的含量测定。Objective： To establish a method for the determination of irisflorentin in Fanggan spray by HPLC. Methods： HPLC method was used to carry on the determination; chromatographic bar was Agilent C18（250 mm×4.6 mm, 5 μm）. The mobile phase was methanol-0.2% phosphoric acid （60 ： 40）. The flow rate was 1.0 mL/min with detection wavelength of 266 nm. Column temperature was set at 30 ℃. Results： The irisflorentin had a good linear relation between 0. 026 - 0. 260 mg/mL （ r = 1. 000 ）. This average recovery was 97.12% and RSD was 1.85%. Conclusions： This method is simple, accurate and reliable. It can be used as a method for determination of irisflorentin in Fanggan spray.

关 键 词：高效液相色谱法 次野鸢尾黄素 防感喷雾剂 

分 类 号：R927.2[医药卫生—药学]