丹酚酸/丹参酮配伍对凝血酶诱导HUVEC细胞炎症因子的影响  

Effect of Salvianolic Acid/tanshinones Compatibility on Thrombin-induced HUVEC Cytokine

作　　者：张萌[1] 孟庆华[1] 朱金墙[1] Orgah John 

机构地区：[1]天津中医药大学

出　　处：《中西医结合心脑血管病杂志》2012年第9期1088-1090,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然科学基金项目(No.30901922)

摘　　要：目的观察丹酚酸/丹参酮配伍对凝血酶诱导人脐静脉内皮细胞(HUVEC)细胞炎症因子P-selection、E-selection的影响。方法培养HUVEC细胞,设空白对照组、凝血酶损伤模型组、丹酚酸/丹参酮配伍50μg/L组、丹酚酸/丹参酮配伍100μg/L组。采用ELISA法检测细胞上清液中P-selection、E-selection含量。结果丹酚酸/丹参酮配伍50μg/L、100μg/L均可显著降低经凝血酶诱导而升高的P-selection含量(P<0.01),丹酚酸/丹参酮配伍100μg/L可显著降低经凝血酶诱导而升高的E-selection含量(P<0.05)。结论丹酚酸/丹参酮配伍可通过抑制炎症因子P-selection、E-selection表达而抗凝血酶诱导的HUVEC炎症损伤。Objective To observe the effect of salvianolic acid/tanshinones compatibility on thrombin induced human umbilical vein endothelial cells （HUVEC） P selection and E selection. Methods HUVEC cells were cultured and divided into blank control group,thrombin injury model group, salvianolic acid/tanshinones 50 μg/L group, salvianolic acid/tanshinones 100 μg/I, group. The contents of P selection and E selection in culture supernatants were detected by ELISA. Results Compared with thrombin injury model group, the content of P selection was decreased in salvianolic aeid/tanshinones 50 μg/L group and salvianolic acid/tanshinones 100 /μg/L group（P〈0.01）. The content of E selection was decreased in salvianolic acid/tanshinones 100 μg/L group（P〈0.05）. Conclusion The satvianolic acid/tanshinones compatibility could ameliorate thrombin induced HUVEC inflammatory injury through inhibition of the inflammatory cytokines P selection and E selection expression.

关 键 词：丹酚酸 丹参酮 凝血酶 人脐静脉内皮细胞 炎症因子 

分 类 号：R285[医药卫生—中药学]