二苯乙烯苷对2型糖尿病大鼠氧化应激、炎症反应及主动脉细胞凋亡相关基因的影响  

Effects of 2,3,4′,5-tetrahydroxystilbene-2-O-beta-D-glucoside on oxidative stress,inflammation and aorta cell apoptosis gene in type 2 diebetes rats

作　　者：张园[1,2] 董林[1] 许晓乐[3] 王玉琴[3] 张伟[3] 

机构地区：[1]南通大学医学院内科学教研室,226001 [2]南通大学医学院附属医院内科 [3]南通大学医学院药理学教研室,226001

出　　处：《中国糖尿病杂志》2012年第10期775-778,共4页Chinese Journal of Diabetes

基　　金：江苏省自然科学基金资助项目(BK2009162);江苏省高校自然科学研究项目(08KJB310008)

摘　　要：目的研究二苯乙烯苷(TSG)对T2DM大鼠氧化应激、炎症反应及主动脉细胞凋亡相关基因的影响,并探讨其可能机制。方法采用高脂喂养联合低剂量STZ(40mg/kg)的方法建立T2DM大鼠模型后,TSG(60、120mg/kg)、辛伐他汀(SV,2mg/kg)灌胃治疗6周。结果与模型组比较,SV治疗组、TSG治疗组超氧化物歧化酶(SOD)活性、HDL-C水平升高(P<0.05),TG、TC、LDL-C、丙二醛(MDA)、FFA、TNF-α、单核细胞趋化蛋白-1(MCP-1)水平降低(P<0.05)。免疫组化测大鼠主动脉核因子κB(NF-κB)、Fas表达下降(P<0.05),Bcl-2表达增强(P<0.05)。结论 TSG对T2DM大鼠有抗氧化、抗炎作用,并能抑制主动脉细胞凋亡相关基因的表达。Objective To discuss the effects of 2,3,4′,5-tetrahydroxystilbene-2-O-beta-D-glucoside（TSG） on oxidative stress,inflammation and aorta cell apoptosis gene in type 2 diabetic rats in vivo,and explore its potential mechanisms.Methods Type 2 diabetes of SD rats was induced by high-fat diet and single intraperitoneal injection of STZ（40 mg/kg）.The successful diabetic rat models were randomized into normal control group,diabetes group,simvastatin（2 mg/kg） in the positive control group,low-and high-dose treatment groups（TSG 60 mg/kg,120 mg/kg）.After 6 weeks’ treatment of gavage,the level of oxidative stress,inflammation and aorta cell apoptosis were measured.Results Compared with the model group,in the SV and TSG groups,the levels of SOD and HDLC increased（P〈0.05）,while the levels of TG,TC,LDLC,MDA,FFA,TNF-α,and MCP-1 decreased（P〈0.05）.Detected by immunohistochemistry in aorta,the expressions of NF-κB and Fas protein were lower（P〈0.05） and expression of Bcl-2 protein was higher（P〈0.05）.Conclusions TSG improves oxidative stress,inhibits inflammation and has anti-apoptotic effect on aorta in the STZ-induced type 2 diabetic rats.

关 键 词：糖尿病 2型 二苯乙烯苷 氧化性应激 炎症 细胞凋亡 

分 类 号：R587.1[医药卫生—内分泌]