一测多评法与外标法测定双黄连制剂中黄酮类成分含量的比较分析  

Simultaneous Determination of Flavones in Shuanghuanglian Preparations by both QAMS and External Standard Method

作　　者：冯伟红[1,2] 杨菲[1,2,3] 王智民[1,2] 张启伟[1,2] 吉丽娜[1,2,3] 李东影[1,2,3] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]中药质量控制技术国家工程实验室,北京100700 [3]天津中医药大学,天津300193

出　　处：《中国药学杂志》2012年第20期1665-1669,共5页Chinese Pharmaceutical Journal

基　　金：国家"重大新药创制"科技重大专项(2009ZX09301-005;2009ZX09308-003);中医药行业科技专业资助项目(200707009)

摘　　要：目的分别采用一测多评法(QAMS)与外标法(ESM)测定双黄连制剂中4种黄酮类成分的含量,以证明一测多评法在复方中应用的可靠性。方法采用RP-HPLC,色谱柱:XtimateTMC18(4.6 mm×250 mm,5μm),流动相:甲醇(A)-0.2%磷酸水溶液(B),梯度洗脱,流速1 mL·min-1,柱温30℃,检测波长274 nm。结果一测多评法的计算值和外标法的实测值无显著差异。结论 "一测多评"法可作为一个新的质量评价模式用于双黄连制剂中黄酮类成分的质量评价。OBJECTIVE To study the feasibility of quantitative analysis of multi-components by single-marker （QAMS） in determination of flavones in Shuanghuanglian preparations by comparing the results assayed by QAMS and external standard method（ESM）. METHODS The analysis was performed on a XtimateTM Cjs column （4. 6 mm ×250 mm,5μm）with methanol and water containing 0. 2% phosphoric acid as the mobile phase in gradient elution. The flow rate was set at 1.0 mL ·min^-1. The UV detector wavelength was 274 nm, and the column temperature was 30 ℃. RESULTS Within the linear ranges, the analysis results showed no significant difference between QAMS and ESM. CONCLUSION The QAMS method is credible and will be a new quality evaluation pattern for the determination of multiple flavones in Shuanghuanglian preparations.

关 键 词：一测多评法 外标法 高效液相色谱法 黄酮 相对校正因子 双黄连制剂 

分 类 号：R917[医药卫生—药物分析学]