附子和蒲公英对乌拉坦诱导肺癌小鼠中医体征的影响  

Effect of Aconiti Lateralis Radix Praeparata and Taraxaci Herba on Chinese medicine signs and symptoms of urethane-induced lung cancer in mice

作　　者：孙婷[1] 杜钢军[1] 张亚平[1] 李佳桓[1] 刘伟杰[1] 王莹莹[1] 

机构地区：[1]河南大学药学院药物研究所,河南开封475001

出　　处：《中国中药杂志》2012年第20期3097-3101,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81173094);河南大学省部共建项目(SBGJ090704);河南省高校青年骨干教师项目(2010GGJS-025);河南省科技厅重点攻关项目(112101310308)

摘　　要：目的:研究乌拉坦诱导肺癌小鼠的中医体征,观察附子和蒲公英对小鼠症状及肿瘤进展的影响。方法:每周2次、连续5周给小鼠腹腔注射乌拉坦建立小鼠肺癌模型,观察诱癌过程中小鼠外观、体温和外耳微循环变化,用能量代谢、红细胞ATP酶活性及血液流变学指标评价乌拉坦诱导肺癌小鼠的中医体征,并在模型建立同时用附子和蒲公英进行治疗,考察其对小鼠症状及肿瘤进展的影响。结果:乌拉坦诱导肺癌过程中小鼠逐渐出现畏寒喜暖、踡缩少动、弓背毛松、体温下降、外耳和尾部血管紫暗现象,同时能量代谢和红细胞ATP酶活性降低,全血黏度及红细胞聚集指数增高。蒲公英对上述体征有增强趋势,但对肿瘤进展无影响;附子可以减弱上述体征、阻止肿瘤进展。结论:乌拉坦诱导的肺癌小鼠表现为寒凝血瘀中医体征,附子治疗能改善小鼠症状和体征,阻止肿瘤进展。Objective： To study Chinese medicine （CM） signs and symptoms of urethane-induced lung cancer in mice, and observe the effect of Aconiti Lateralis Radix Praeparata and Taraxaci Herba on symptoms in mice and tumor progress. Method： The mice were intraperitoneally injected with urethane twice a week for consecutively five weeks to establish a lung cancer model. The chan- ges in their appearance, body temperature and auricle microcirculation were observed in carcinogenic process. CM signs and symptoms of urethane-induced lung cancer in mice were evaluated with energy metabolism, erythrocytic ATP emzymatic activity and hemorrheo- logical index. During the tumor model was induced, Aconiti Lateralis Radix Praeparata and Taraxaci Herba were used to treat the mice and observe their effect on symptoms in mice and tumor progress. Result： During urethane was used to induce lung cancer, the mice had gradually become chill, lazy, hunched, with reduction in temperature, cyanosis in auricle and tail. Meanwhile, their energy me- tabolism and erythrocytic ATP enzymatic activity reduced, whereas their whole blood viscosity and erythrocytic aggregate index in- creased. Taraxaci Herba showed an effect on enhancing above symptoms and signs but had no effect on tumor progress. Aconiti Latera- 1is Radix Praeparata showed an effect on reducing above symptoms and signs and preventing tumor progress. Conclusion： Mice with urethane-induced lung cancer show CM signs and symptoms of congealing cold with blood stasis. The treatment with Aconiti Lateralis Radix Praeparata can alleviate symptoms and signs in mice and prevent tumor progress.

关 键 词：附子 蒲公英 乌拉坦 肺癌 中医体征 

分 类 号：R285[医药卫生—中药学]