采用接触式培养皿法对采血人员手部金黄色葡萄球菌与大肠埃希菌的检测效果分析  

Contact petri dish in detection of Staphylococcus aureusand Escherichia coli on hands of blood collection staff

作　　者：赵安成[1] 

机构地区：[1]临沂市沂水中心医院检验科,山东临沂276400

出　　处：《中华医院感染学杂志》2012年第20期4673-4674,共2页Chinese Journal of Nosocomiology

摘　　要：目的评价接触式培养皿法对采血人员手部金黄色葡萄球菌与大肠埃希菌的检测效果。方法将28名采血人员在穿刺采血之前的手部消毒前后,根据采取的消毒方式不同分为两组,其中接触式培养皿组采用接触式培养皿法,棉签擦拭组采用棉签擦拭法进行,分别取样、培养、菌落计数、用金黄色葡萄球菌和大肠埃希菌鉴别培养基鉴定该种类菌株数。结果接触式培养皿法消毒前细菌总数>30CFU/30cm2的人数占50.00%,显著高于棉签擦拭法的7.13%,消毒后细菌总数在21～30CFU/30cm2接触式培养皿法检测出人数占14.29%,棉签擦拭法则未检出(P<0.05);消毒前后接触培养皿组采血人员手部消毒前后金黄色葡萄球菌与大肠埃希菌的检出量均显著高于棉签擦拭组(P<0.05)。结论接触式培养皿法在检测菌落总数及金黄色葡萄球菌、大肠埃希菌均具有较高的有效性,且操作简便。OBJECTIVE To evaluate the effect of contact petri dish on the detection of Staphylococcus aureus and Escherichia coli in the hands of the blood collection staff.METHODS A total of 28 cases of blood collection staff were divided into two groups according to the disinfection methods before the puncture for the blood sampling.The contact petri dish group was taken with contact petri dish method,and the swab group was taken with the swab method.The number of the species of pathogens were determined by sampling,culture,colony counting,and identification culture medium for S.aureus and E.coli.RESULTS The blood collection staff in the contact petri dish group before the disinfection,with the total bacterial colony counts more than 30 CFU/30 cm2,accounted for 50.00%,significantly higher than 7.13% of the swap group.The blood collection staff in the contact petri dish group after the disinfection,with the bacterial colony counts 21-30 CFU/30 cm2,accounted for 14.29%,there were no strains of pathogens detected in the swab group（P0.05）;before and after disinfection,the volume of S.aureus and E.coli detected by the contact petri dish before and after the disinfections was significantly higher than that by the swab（P0.05）.CONCLUSION The contact petri dish method is effective and simple in the detection of the total bacterial colonies counts of S.aureus and E.coli.

关 键 词：接触式培养皿 手卫生 金黄色葡萄球菌 大肠埃希菌 

分 类 号：R378[医药卫生—病原生物学]