儿童难治性癫痫外周血多药耐药相关蛋白1表达研究  

Study of MRP 1 expression in peripheral blood of children with refractory epilepsy

作　　者：岳璇[1] 刘晓鸣 袁宝强[1] 李瑞 

机构地区：[1]徐州医学院附属医院儿科,江苏徐州221000 [2]徐州市儿童医院,江苏徐州221000

出　　处：《中国实用儿科杂志》2012年第10期774-776,共3页Chinese Journal of Practical Pediatrics

摘　　要：目的研究儿童难治性癫痫外周血多药耐药相关蛋白1(MRP1)的表达情况。方法 2010年11月至2011年10月徐州市儿童医院门诊及住院收治的癫痫患儿60例,分为难治性癫痫组和药物控制良好组,各30例;选取同期来院体检的健康儿童30名为对照组。采用逆转录酶聚合酶链式反应(RT-PCR)和蛋白免疫印迹(Westernblot)方法测定3组外周血MRP1mRNA及蛋白含量。结果难治性癫痫组MRP1表达明显高于药物控制良好癫痫组及对照组,差异均有统计学意义(P<0.05);药物控制良好癫痫组与对照组比较差异无统计学意义(P>0.05)。结论难治性癫痫患儿外周血MRP1表达增强。Objective To investigate the expression of muhidrug resistant-associated protein 1 in the peripheral blood of children with refraeotory epilepsy. Methods The expression of MRP1 was detected by RT-PCR and Western blot in children with intractable epilepsy, epilepsy controlled by anti-epileptic drugs （AEDs） and healthy children.Results The two methods showed that MRP1 expression in intractable epilepsy was higher than that in epilepsy controlled by an- ti-epileptic drugs （AEDs） and healthy children（P 〈 0.05）, and there was no difference between epilepsy controlled by anti-epileptic drugs （AEDs） and healthy children.Conclusion MRP1 overexpresses in peripheral blood of children with refractory epilepsy.

关 键 词：难治性癫痫 多药耐药相关蛋白1 儿童 外周血 

分 类 号：R72[医药卫生—儿科]