EDC/NHS交联改性再生桑蚕丝素纳米纤维  

EDC/NHS Crosslinking Modification of Regenerated Silk Fibroin Nanofiber

作　　者：明津法[1,2] 刘蓉[2] 张焕相[3] 左保齐[1,2] 

机构地区：[1]苏州大学现代丝绸国家工程实验室,江苏苏州215123 [2]苏州大学纺织与服装工程学院,江苏苏州215021 [3]苏州大学基础医学与生命科学学院,江苏苏州215123

出　　处：《高分子材料科学与工程》2012年第10期162-165,169,共5页Polymer Materials Science & Engineering

基　　金：国家自然科学基金资助项目(30671041;30870642);江苏高校优势学科建设工程资助项目(苏政办发[2011]6号);苏州市科技计划项目(SG201046)

摘　　要：用静电纺丝技术制备了再生桑蚕丝素纳米纤维,并用1-(3-二甲基氨基丙基)-3-乙基碳化二亚胺(EDC)和N-羟基丁二酰亚胺(NHS)进行交联改性,考察了交联改性前后,桑蚕丝素纳米纤维微观形貌及聚集态结构等的变化,采用扫描电镜(SEM)、X射线衍射(XRD)及红外光谱(FT-IR)等测试方法对纳米纤维进行表征。研究结果表明,经EDC/NHS交联改性后,纤维直径由250 nm增加到320 nm,纤维表面变粗糙,且纤维发生弯曲变形;丝素的结构以Silk II为主,并含有部分无规卷曲或Silk I构象;桑蚕丝纳米纤维的力学性能和亲水性有所提高,且交联改性后的纳米纤维具有良好的细胞相容性。Regenerated silk fibroin nanofiber prepared by electrospinning methods has crosslinked with 1-（3-dimethylaminopropyl）-3-ethylcarbodiimide/N-hydroxy succinimide（EDC/NHS）.The morphology of uncrosslinked and crosslinked silk fibroin nanofiber mats was studied by SEM,and its aggregated structure was characterized by X-ray diffraction and FT-IR analysis et al.The results show that after crosslinking,the average diameter of crosslinked nanofiber increases from 250 nm to 320 nm;the crosslinked silk fibroin nanofiber deforms and its surface becomes rough;the porosity of silk fibroin nanofiber mats decreases.At the same time,the secondary structure of crosslinked silk fibroin nanofiber is mainly in Silk II,containing partial random coil or Silk I conformation.The crosslinked silk fibroin nanofiber has better mechanical properties and hydrophilicity than the uncrosslinked materials.In addition,the crosslinked silk fibroin nanofiber shows good biocompatibility.

关 键 词：桑蚕丝素 静电纺丝 交联 红外光谱 

分 类 号：TB383[一般工业技术—材料科学与工程]