地高辛标记人TGFβ_1cDNA探针的制备  

The preparation of digoxin labeled cDNA probe of human TGF β_1

作　　者：祁赞梅[1] 翟玲[1] 吕昌龙[1] 阎建中 赛红[2] 

机构地区：[1]中国医科大学免疫学教研室,辽宁沈阳110001 [2]大连医科大学检验系免疫学教研室,辽宁大连116027

出　　处：《大连医科大学学报》2000年第2期145-146,共2页Journal of Dalian Medical University

摘　　要：用EcoRI/XhoI两种限制性内切酶在同一反应体系中对TGFβ1 cDNA重组质粒联合酶切获得 1 3Kb的酶切片段作为裸探针 ,比以往文献中报道的长度缩短约 70 0bp,经地高辛标记获得较高的标记效率。本方法简化了操作步骤 ,并大大降低了cDNA探针的制备成本 ,不失为制备TGFβ1 cDNA探针的好方法 ,为TGFβ1 mRNA的检测提供了较好的工具。In this study we used a 1 3 Kb long DNA fragment as nude probe which was obtained from the recombinant plasmid of TGF β 1cDNA with the combine incision of two endonuclease EcoR I/Xho I in the same reaction system.This probe is shorter by 700 bp than those early reported and is labeled by digoxin with high efficiency.This method not only simplified the operation steps,but also reduced the cost of the preparation of cDNA probe.therefore,it can be regarded as a good measure for the preparation of TGF β 1cDNA probe,and provides a good mean for the detection of TGF β 1mRNA. [WT5HZ]

关 键 词：地高辛标记 TGFβcDNA探针 制备 

分 类 号：R392.1[医药卫生—免疫学]